AI Article Synopsis
- Biosensors using surface plasmon resonance (SPR) allow for real-time, label-free analysis of biomolecular interactions, making them effective for studying various biological processes like antibody-antigen interactions.
- SPR-based biosensors enable the separate determination of association and dissociation rate constants, offering more detailed kinetic analysis than traditional methods.
- The text outlines the hardware used in commercial SPR systems, provides practical procedures for use, and discusses the technology's potential applications in drug design and therapeutic antibody development, along with its limitations.
Article Abstract
The use of biosensors employing surface plasmon resonance (SPR) provides excellent instrumentation for a label-free, real-time investigation of biomolecular interactions. A broad range of biological applications including antibody-antigen interactions can be analyzed. One major advantage of kinetic analysis using SPR-based biosensors is the option of determining separately distinct association and dissociation rate constants exceeding the classical steady-state analysis of biomolecules. Based on these data new possibilities for drug design, characterizing human pathogens, and the development of therapeutic antibodies can be achieved. The hardware of commercially available systems is described, practical step by step procedures are given, and possibilities and limitations of the technology are discussed.
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| http://dx.doi.org/10.1385/1-59259-679-7:299 | DOI Listing |
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