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Decellularization of porcine corneas and repopulation with human corneal cells for tissue-engineered xenografts.
Acta Ophthalmol
March 2012
Department of Ophthalmology, Eberhard-Karls University, 72076 Tuebingen, Germany.
Purpose: To evaluate the potential use of decellularized porcine corneas (DPCs) as a carrier matrix for cultivating human corneal cells in tissue engineering.
Methods: Corneal cells were isolated from human corneoscleral rims. Porcine corneas were decellularized using hypotonic tris buffer, ethylene diamine tetra-acetic acid (EDTA, 0.
Antihypertension and anti-cardiovascular remodeling by phenylalanine in spontaneously hypertensive rats: effectiveness and mechanisms.
Chin Med J (Engl)
March 2001
Ruijin Hospital, Shanghai Second Medical University, Shanghai Institute of Hypertension, Shanghai 200025, China.
Objective: To investigate mechanisms of anti-hypertension and anti-cardiovascular remodeling by phenylalanine (phe) in spontaneously hypertensive rats (SHRs).
Methods: The comparison of blood pressure (BP) increment with the ages and cardiovascular changes of SHRs was made between the 3% phe-intervented group (SHR-phe) and the control SHRs group. Detection of the structural changes with the VIDAS digital vedio-frequency processing technique and light and electron microscopy were made.
Immune response in aged and young subjects, following administration of large doses of tuberculin.
Int Arch Allergy Appl Immunol
August 1975
Different parameters of the immunologic response have been studied in young and aged subjects before, during and after administration of increasing doses of tuberculin purified protein derivative (PPD). In both age groups, a considerable increase in skin reaction was observed with the progression of antigen administration. This is particularly striking among old subjects, who before the treatment had either no reaction to tuberculin or a very weak one.
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