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Development of an accurate and sensitive assay for 2-methoxyestradiol using derivatization and liquid chromatography-tandem mass spectrometry.
Pract Lab Med
April 2025
Laboratories Division, Shimane University Hospital, Shimane, Japan.
2-Methoxyestradiol (2ME) is involved in the pathogenesis of preeclampsia and antitumor activity. In addition to its low concentration in healthy human serum, presence of isomers makes quantification of 2ME for clinical research and laboratory medicine difficult. The objective of this study was to develop a highly sensitive and accurate method for quantifying 2ME using LC-MS/MS combined with derivatization with 1-(2,4-dinitro-5-fluorophenyl)-4,4-dimethylpiperazinium iodide (MPDNP-F).
View Article and Find Full Text PDFLigand guided in vivo crosslinking and affinity purification mass spectrometry for identifying membrane receptors of Tau.
Talanta
January 2025
State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Misfolded neurotoxic proteins, such as Tau protein, spread within the brain in many neurodegenerative diseases. Receptors play an important role in the recognition of spreading proteins for endocytosis. Blocking the receptors is essential to inhibit neurotoxic proteins spreading in the brain.
View Article and Find Full Text PDFPreparation of the immobilized α-adrenoceptor column by the ultra-high affinity protein pair CL7/Im7 and its application in drug-protein interaction analysis.
J Chromatogr B Analyt Technol Biomed Life Sci
January 2025
Northwest University Chang An Hospital, Faculty of Life Sciences and Medicine, Northwest University, Xi'an, Shaanxi 710069, China; Department of Clinical Pharmaceutics, Chang An District Hospital, Xi'an, Shaanxi 710118, China. Electronic address:
Immobilizing the target protein on a solid surface with controlled orientation, high specificity, and maintained activity is a proven strategy to enhance the stability of the protein. In this study, we employed an ultra-high affinity protein pair consisting of a mutant of colicin E7 Dnase and its corresponding inhibitor, immunity protein 7(Im7), to develop an immobilized α-adrenoceptor (α-AR) column. Briefly, we expressed α-AR fused with CL7 as a tag at its C-terminus in Escherichia coli cells.
View Article and Find Full Text PDFReview of pretreatment and analytical methods for environmental endocrine disruptors: Phthalates.
Environ Toxicol Chem
January 2025
School of Energy and Environmental Engineering, University of Science and Technology Beijing, 100083China.
Phthalates, known as phthalate esters (PAEs), are among the most ubiquitous pervasive env7ironmental endocrine disruptors (EEDs), extensively utilized globally in various facets of modern life due to their irreplaceable role as plasticizers. The exponential production and utilization of plastic goods have substantially escalated plastic waste accumulation. Consequently, PAEs have infiltrated the environment, contaminating food and drinking water reservoirs, posing notable threats to human health.
View Article and Find Full Text PDFBackground Thiopurine metabolites, 6-thioguanine (6TG) and 6-methylmercaptopurine (6MMP), are monitored to aid therapeutic management of thiopurine drugs. At Manchester University NHS Foundation Trust (MFT), thiopurine metabolites are measured by high performance liquid chromatography with ultraviolet detection (HPLC-UV). Whole blood samples are lysed and subjected to hydrolysis with derivatisation of 6MMP before HPLC-UV detection at 304nm for the 6MMP-derivative and 342nm for 6TG.
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