Electrofusion was employed for hybrid construction in ergosterol-producing yeast strains. Some fusion products proved to be hybrid with respect to ergosterol content and to remain stable over several generations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jobm.3620320604 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sterol Biosynthesis in Four Green Algae: A Bioinformatic Analysis of the Ergosterol Versus Phytosterol Decision Point.
J Phycol
August 2021
School of Biological Sciences, University of Nebraska, Lincoln, Nebraska, 68588-0666, USA.
Animals and fungi produce cholesterol and ergosterol, respectively, while plants produce the phytosterols stigmasterol, campesterol, and β-sitosterol in various combinations. The recent sequencing of many algal genomes allows the detailed reconstruction of the sterol metabolic pathways. Here, we characterized sterol synthesis in two sequenced Chlorella spp.
View Article and Find Full Text PDFIn vivo direct interaction of the antibiotic primycin on a Candida albicans clinical isolate and its ergosterol-less mutant.
Acta Biol Hung
March 2012
Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Hungary.
Interaction of primycin antibiotic with plasma membrane, and its indirect biological effects were investigated in this study. The antifungal activity of primycin against 13 human pathogenic Candida ATCC and CBS reference species and 74 other Candida albicans clinical isolates was investigated with a microdilution technique. No primycin-resistant strain was detected.
View Article and Find Full Text PDFDirect in vivo interaction of the antibiotic primycin with the plasma membrane of Candida albicans: an EPR study.
Biochim Biophys Acta
January 2012
Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
The direct interaction of the antibiotic primycin with the plasma membrane was investigated by employing the well-characterized ergosterol-producing, amphotericin B-sensitive parental Candida albicans strain 33erg(+) and its ergosterol-less amphotericin B-resistant plasma membrane mutant erg-2. The growth inhibition concentration in shaken liquid medium was 64 μgml(-1) for 33erg(+) and 128 μgml(-1) for erg-2, suggesting that the plasma membrane composition influences the mode of action of primycin. To determine the primycin-induced changes in the plasma membrane dynamic, electron paramagnetic resonance (EPR) spectroscopy methods were used, the spin-labeled fatty acid 5-(4,4-dimethyloxazolidine-N-oxyl)stearic acid) being applied for the in vivo measurements.
View Article and Find Full Text PDFConstruction of high ergosterol-producing yeast strains and study on the optimal conditions for culture.
Chin J Biotechnol
April 2000
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
High ergosterol-producing yeast strains were constructed by primary screening isolation of haploid mutagenesis and protoplasts fusion. The fermentation conditions of fused strain YEF-21 were studied. Under the optimal conditions, i.
View Article and Find Full Text PDFIn vivo interaction of trivalent chromium with yeast plasma membrane, as revealed by EPR spectroscopy.
FEMS Microbiol Lett
January 2000
Department of Genetics and Microbiology, Janus Pannonius University, Ifjúság u. 6., P.O. Box 288, H-7624, Pécs, Hungary.
The in vivo effects of CrCl(3) on an ergosterol-producing 33 erg(+) strain of the eukaryotic yeast Candida albicans, and on its ergosterol-deficient erg(-)2 mutant, were studied by using electron paramagnetic resonance spectroscopy. A 5-doxylstearic acid spin probe was applied to label the membranes. The absence of ergosterol, an increased accumulation of Delta(8) sterols, a decreased fatty acid chainlength and a lower proportion of unsaturated fatty acids of the erg(-)2 mutant resulted in a higher membrane rigidity and an increased sensitivity to Cr(III) than those of the parental 33 erg(+) strain.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!