Proteins of a crude enzyme preparation obtained from the cultivation medium of the basidiomycete Phellinus abietis were separated by gel filtration and ion-exchange chromatography. The preparation contained a minimum of three enzymes capable of splitting alpha-D-mannosidic bonds: alpha-mannosidase, exomannanase, and endomannanase, which were separated. Some properties of the mannanase complex of the crude enzyme preparation, and of a partially purified alpha-mannosidase were examined. The mannanase complex exhibited two pH optima, its temperature optimum being at 45 degrees C. The pH optimum of purified alpha-mannosidase was at pH 5.0, the temperature optimum being at 45 degrees C. The pH optimum of purifed alpha-mannosidase was at pH 5.0, the temperature optimum at at 60 degrees C; the enzyme had a relatively high heat stability. The Km of alpha-mannosidase for p-nitrophenyl alpha-D-mannopyranoside was 1.5 X 10(-5) M. Pure alpha-mannosidase did not split mannan.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02881634 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimizing Soil Stabilization with Chitosan: Investigating Acid Concentration, Temperature, and Long-Term Strength.
Polymers (Basel)
January 2025
College of Science, Australia National University, Canberra, ACT 2600, Australia.
Civil and geotechnical researchers are searching for economical alternatives to replace traditional soil stabilizers such as cement, which have negative impacts on the environment. Chitosan biopolymer has shown its capacity to efficiently minimize soil erosion, reduce hydraulic conductivity, and adsorb heavy metals in soil that is contaminated. This research used unconfined compression strength (UCS) to investigate the impact of chitosan content, long-term strength assessment, acid concentration, and temperature on the improvement of soil strength.
View Article and Find Full Text PDFRefining Surface Copper Species on Cu/SiO Catalysts to Boost Furfural Hydrogenation to Furfuryl Alcohol.
Molecules
January 2025
College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
Controllable hydrogenation of carbonyl groups (C=O) is crucial for converting furfural into high-value furfuryl alcohol. Instead of traditional impregnation method, a novel Cu-based catalyst (Cu/SiO) is prepared using the ammonia evaporation method (AE) for the efficient hydrogenation of furfural to furfuryl alcohol under mild conditions. At the reaction conditions of 90 °C and 1 MPa H, the 5Cu/SiO-AE sample showed optimal performance with higher turnover frequency (36.
View Article and Find Full Text PDFSO Removal from Flue Gas by Char-Supported Fe-Zn-Cu Sorbent.
Materials (Basel)
January 2025
Key Laboratory for Advanced Coal and Coking Technology of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China.
In this study, the mechanisms of SO adsorption on lignite char and char-supported Fe-Zn-Cu sorbent (FZC sorbent) were investigated. The FZC sorbent was prepared by the impregnation of metal components on raw coal followed by steam gasification. Flue gas desulfurization experiments were carried out on a fixed-bed reactor at 100-300 °C by using simulated flue gas containing SO/O/HO balanced by N.
View Article and Find Full Text PDFFormation of a Novel Antagonistic Bacterial Combination to Enhance Biocontrol for Cucumber Wilt.
Microorganisms
January 2025
Institute of Vegetable, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China.
strain PJH16, isolated and tested by our team, suppresses cucumber wilt as an efficient biocontrol agent. For further investigation, the strain has been combined with two other ( VJH504 and JNF2) to enhance biocontrol ability, which formed high-efficiency microbial agents in the current study. The methodological target taken is based on achieving the optimal growth conditions of the combined microbial agents; hence, the medium composition and culture conditions were optimized through a single-factor test, orthogonal test and response surface methodology.
View Article and Find Full Text PDFMitigation of Mycotoxin Content by a Single-Screw Extruder in Triticale ( Wittmack).
Foods
January 2025
Institute of Food Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
The aim of this study was to investigate the effects of extrusion processing parameters-moisture content ( = 20 and 24%), feeding rate ( = 20 and 25 kg/h), and screw speed ( = 300, 390 and 480 RPM), on the content of deoxynivalenol (DON), 15-Acetyl Deoxynivalenol (15-AcDON), 3-Acetyl Deoxynivalenol (3-AcDON), HT-2 Toxin (HT-2), tentoxin (TEN) and alternariol monomethyl ether (AME), using a pilot single-screw extruder in whole-grain triticale flour. The temperature at the end plate of the extruder ranged between 97.6 and 141 °C, the absolute pressure was from 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!