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Understanding Folding of bFGF and Potential Cellular Protective Mechanisms of Neural Cells.
Biochemistry
January 2025
Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, Maryland 20742, United States.
Article Synopsis
- Traumatic brain injury (TBI) affects many individuals, especially veterans and athletes, and has serious, long-term consequences for brain health.
- Current research explores the role of fibroblast growth factor (FGF) proteins in protecting cells, highlighting knowledge gaps regarding how heparin and similar molecules activate bFGF and how mutations affect its stability.
- Using temperature replica exchange, the study identified a new binding site on bFGF and revealed that various sugars affect bFGF interactions similarly to heparin, underscoring the need for a deeper understanding of TBI mechanisms for better treatment development.
Activation of the Contact System and Intrinsic Pathway in Peripheral and Portal Venous Circulations in Liver Cirrhosis.
Thromb Haemost
January 2025
Internal Medicine 1, Thrombotic and Hemorrhagic Diseases Unit, Department of Medicine, Padova University Hospital, Padova, Italy.
Background: Portal vein system-specific risk factors contributing to portal vein thrombosis in cirrhosis are poorly investigated.
Aim: This study aimed to quantify contact system and intrinsic pathway activation in the peripheral compared to portal venous blood in patients with decompensated cirrhosis.
Methods: Adult patients with cirrhosis undergoing transjugular intrahepatic portosystemic shunt underwent simultaneous blood sampling from a peripheral vein and the portal vein.
Thromboelastography for rapid diagnosis of heparin-like anticoagulant release during anaphylaxis-induced coagulopathy in systemic mastocytosis: a case report.
Clin Med (Lond)
November 2024
King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia; Department of Medicine King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.
Anaphylaxis can induce life-threatening coagulopathy by releasing various mediators from activated mast cells. These mediators directly affect coagulation and fibrinolytic pathways, increasing the bleeding risk. Diagnosis and management of anaphylaxis-induced coagulopathy remain challenging.
View Article and Find Full Text PDFFunctionally distinct anticoagulant mechanisms of endothelial cells.
Thromb Res
December 2024
Synapse Research Institute Maastricht, Kon. Emmaplein 7, 6217, KD, Maastricht, the Netherlands.
Antithrombin and tissue factor pathway inhibitor (TFPI) provide different anticoagulant mechanisms. Having established a potent anticoagulant role of cultured human umbilical vein endothelial cells in vessel-on-a-chip microfluidic models, we now investigated how these cells modulated thrombin generation under stasis through antithrombin and TFPI pathways. We observed that endothelial monolayers in 96 well-plates strongly delayed and suppressed the thrombin generation process induced by tissue factor, regardless of the presence of whole blood, platelet-rich plasma or platelet-free plasma.
View Article and Find Full Text PDFHighly pure measles virus generated by combination of salt-active nuclease treatment and heparin affinity chromatography.
J Chromatogr A
December 2024
Austrian Centre of Industrial Biotechnology, Vienna, Austria; Institute of Bioprocess Science and Engineering (IBSE), BOKU University, Vienna, Austria.
Article Synopsis
- Highly purified virus preparations are crucial for determining the activity and potency of viruses, necessitating simple and efficient purification methods in early research phases.* -
- The study focused on using heparin affinity chromatography for purifying a sensitive strain of the measles virus, achieving high capture rates of infectious virus while experimenting with endonuclease treatments to reduce DNA impurities.* -
- The combination of Capto™ Heparin with M-SAN endonuclease resulted in a notable yield of 62% purity and significantly lower DNA contamination, suggesting this method is suitable for measles virus production in a scalable manufacturing process.*
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