Objective: To investigate the mechanism of cytokeratin 13 (CK13) gene expression control and the effects of different motifs of CK13 gene 5' flanking region on its transcriptional activity.
Methods: The molecular clone technique and reporter gene analysis were used to assay the effects of different motifs of 513 bp of CK13 gene 5' flanking region on its transcriptional activity. The pCAT enhancer vectors with different motifs of CK13 gene 5' flanking region were constructed and transferred to HeLa cells with the help of lipofectin. The instant CAT expression of different clones was detected and the effects of different motifs of the CK13 gene 5' flanking region on its transcriptional activity were evaluated.
Results: 119 bp from -nt.325 to -nt.207 upstream of the first ATG of CK13 gene 5' flanking region included a silent element. 113 bp region from -nt.206 to -nt.94 included an enhanced element.
Conclusion: 513 bp of CK13 gene 5' flanking region includes a silent element and an enhanced element. Further locating these cis elements and detecting the related trans reaction factors may unveil some important clues to the details of the mechanisms for the CK13 gene expression and tissue-specific expression.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Wnt/β-catenin-YAP axis in the pathogenesis of primary intraosseous carcinoma NOS, deriving from odontogenic keratocyst.
Pathol Res Pract
August 2024
Laboratory of Oral Pathology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Article Synopsis
- Odontogenic tumors (OGTs) are rare, and primary intraosseous carcinoma NOS (PIOC) is an even rarer subtype with a poor prognosis, characterized by unclear immunohistochemical features and pathogenesis.
- A histopathological study of a PIOC case in the left mandible revealed a transition from an odontogenic keratocyst to carcinoma, with signs of malignant behavior such as invasive growth and specific biomarker alterations.
- Immunohistochemical analysis indicated increased levels of certain proteins (ARL4C, YAP) in the tumor, activation of Wnt/β-catenin signaling, and suggested that varied intracellular signaling pathways might play a role in the disease's pathogenesis.
The increased expression of cytokeratin 13 leads to an increase in radiosensitivity of nasopharyngeal carcinoma HNE-3 cells by upregulating ERRFI1.
IUBMB Life
August 2023
Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated Hospital of Yunnan University, the Second People's Hospital of Yunnan Province, Yunnan Eye Hospital, Kunming, Yunnan, People's Republic of China.
The main factors contributing to the unfavorable outcome in the clinical treatment of patients with nasopharyngeal carcinoma (NPC) patients are radiation resistance and recurrence. This study aimed to investigate the sensitivity and molecular foundation of cytokeratin 13 (CK13) in the radiotherapy of NPC. To achieve this, a human NPC cell line overexpressing CK13, HNE-3-CK13, was constructed.
View Article and Find Full Text PDFsp. nov., isolated from a tidal flat sediment of the East China Sea.
Int J Syst Evol Microbiol
May 2022
State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, PR China.
A Gram-stain-negative bacterial strain, designated as E165, was isolated from a tidal flat sediment of the East China Sea. Strain E165 grew optimally at pH 6, at 32 °C and with 1-2 % (w/v) NaCl. The 16S rRNA gene sequence similarity results revealed that strain E165 was most closely related to IVIA-Po-145, CK13, IVIA-Po-155, HDW-15, 46-16, B20-1 and DSL-35 with values of 97.
View Article and Find Full Text PDFCytokeratin 13 promotes radiotherapy sensitivity of nasopharyngeal carcinoma by downregulating the MEK/ERK pathway.
IUBMB Life
June 2022
Department of Otorhinolaryngology, Head and Neck Surgery, The Affiliated Hospital of Yunnan University, the Second People's Hospital of Yunnan Province, Yunnan Eye Hospital, Kunming, China.
Background: Radiation therapy is the first treatment choice for nasopharyngeal carcinoma (NPC), while radiation resistance and recurrence have become the primary factors and are associated with poor prognosis in the clinical treatment of NPC patients. The purpose of the present study was to explore the sensitivity and molecular basis of cytokeratin 13 (CK13) that regulates NPC radiotherapy.
Methods: HNE-3 or C666-1 cell line was used for overexpression and knockdown tests.
Central mucoepidermoid carcinoma arising directly from a glandular odontogenic cyst of the mandible: a case report.
Diagn Pathol
July 2021
Division of Oral Pathology, Department of Tissue Regeneration and Reconstruction, Faculty of Dentistry, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkoucho-dori, Chuo-ku, 951-8514, Niigata, Japan.
Background: Central mucoepidermoid carcinoma (MEC) is a rare salivary gland tumor that affects the jawbone. Glandular odontogenic cyst (GOC) is also a rare odontogenic developmental cyst with glandular differentiation. GOC shares some histological features with central MEC, and a pre-existing GOC can develop into central MEC.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!