AI Article Synopsis
- Dysregulation of TACC genes is linked to the development of cancers like multiple myeloma, breast, and gastric cancer, but their functional mechanisms remain unclear.
- Researchers have discovered that the histone acetyltransferase hGCN5L2 interacts with TACC2 using yeast two-hybrid assays and further confirmed this interaction via GST pull-down and coimmunoprecipitation in human cells.
- This interaction suggests that abnormal TACC protein expression may influence gene regulation and chromatin remodeling, potentially contributing to cancer progression.
Article Abstract
Dysregulation of the human transforming acidic coiled coil (TACC) genes is thought to be important in the development of multiple myeloma, breast and gastric cancer. However, even though these proteins have been implicated in the control of cell growth and differentiation, the mechanism by which they function still remains to be clarified. Using the yeast two-hybrid assay, we have now identified the histone acetyltransferase (HAT) hGCN5L2 as a TACC2-binding protein. GST pull-down analysis subsequently confirmed that all human TACC family members can bind in vitro to hGCN5L2. The authenticity of these interactions was validated by coimmunoprecipitation assays within the human embryonic kidney cell line HEK293, which identified the TACC2s isoform as a component consistently bound to several different members of HAT family. This raises the possibility that aberrant expression of one or more TACC proteins may affect gene regulation through their interaction with components of chromatin remodeling complexes, thus contributing to tumorigenesis.
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| http://dx.doi.org/10.1038/sj.onc.1207424 | DOI Listing |
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