Novel method using a laser scanning cytometer for detection of mycobacteria in clinical samples.

J Clin Microbiol

Department of Microbiology, Faculty of Medicine, University of Porto, Hospital S João, Porto, Portugal.

Published: February 2004

In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect MYCOBACTERIA:

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC344451PMC
http://dx.doi.org/10.1128/JCM.42.2.906-908.2004DOI Listing

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