Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC348918 | PMC |
| http://dx.doi.org/10.1128/AEM.70.2.1238-1241.2004 | DOI Listing |
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