AI Article Synopsis
- The rat Hst70 gene and mouse Hsp70.2 are specifically expressed in certain stages of sperm cell development.
- A segment of the Hst70 gene promoter can effectively drive testis-specific expression of a reporter gene in mice, mimicking the expression pattern of the Hsp70.2 gene.
- Additional regulatory sequences are necessary for high-level tissue-specific expression, and certain proteins bind to the Oct sequence in juvenile rat testes when Hst70 expression is suppressed.
Article Abstract
The rat Hst70 gene and its mouse counterpart Hsp70.2 are expressed specifically in pachytene primary spermatocytes and spermatids. Here we demonstrate that a 165 bp fragment of the Hst70 gene promoter, containing the T1 transcription start site region, entire exon 1 and 42 bp 5' region of the intron, is sufficient to drive testis-specific expression of the chloramphenicol acetyltransferase reporter gene in transgenic mice with the same developmentally regulated pattern as the endogenous Hsp70.2 gene. We show further that high-level tissue-specific gene expression requires additional sequences localized upstream of the T2 transcription start site. Electrophoretic mobility-shift assay analysis revealed that only testes of juvenile rats, when Hst70 gene expression is repressed, contain proteins that specifically bind to the Oct (octamer) sequence localized directly downstream of the T1 site.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1224129 | PMC |
| http://dx.doi.org/10.1042/BJ20031842 | DOI Listing |
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