The general population is exposed to phthalates through consumer products, diet, and medical devices. The present study explored whether phthalates, reproductive toxins in laboratory animals, were associated with altered sperm movement characteristics in men. Two-hundred twenty subjects provided a semen sample for computer-aided sperm analysis (CASA) and a urine sample for measurement of phthalate monoesters, monoethyl (MEP), monobenzyl (MBzP), mono-n-butyl (MBP), mono-2-ethylhexyl (MEHP), and monomethyl (MMP). Three CASA parameters, straight-line velocity (VSL), curvilinear velocity (VCL), and linearity (LIN), were used as measures of sperm progression, sperm vigor, and swimming pattern, respectively. There were suggestive dose-response relationships (shown as the predicted change in mean sperm motion parameter for the second and third tertiles compared with the first tertile; P value for trend) for MBzP with VSL (-2.36 microm/s, -2.81 microm/s; P =.09) and VCL (-1.67 microm/s, -2.45 microm/s; P =.4). There were suggestive negative associations between MBP and VSL (-3.07 microm/s, -2.87 microm/s; P =.08) and VCL (-3.25 microm/s, -3.46 microm/s; P =.2), and between MEHP with VSL (-1.09 microm/s, -2.73 microm/s; P =.1) and VCL (-0.29 microm/s, -2.93 microm/s; P =.3). In contrast to the other phthalates, MEP was positively associated with VSL and VCL but negatively associated with LIN. No consistent relationship was found for MMP and any sperm motion parameter. Although we did not find statistically significant associations, trends between CASA parameters, sperm velocity, and forward progression, and increased urinary levels of MBP, MBzP, and MEHP warrant further follow-up.
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http://dx.doi.org/10.1002/j.1939-4640.2004.tb02790.x | DOI Listing |
Zhonghua Nan Ke Xue
October 2013
Department of Reproductive Healthcare, Shenzhen Bao'an Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518133, China.
Objective: To investigate the effects of hepatitis B virus (HBV) in semen on human semen parameters and sperm DNA integrity.
Methods: We detected HBV DNA in the semen samples of 153 HBsAg-seropositive patients by real-time fluorescence quantitative PCR and calculated the sperm nuclear DNA fragmentation index (DFI) by sperm chromatin dispersion (SCD) assay. We compared the semen parameters between the HBV DNA-positive group (A, n = 43) and HBV DNA-negative group (B, n = 110) and analyzed the correlation of sperm DFI with the number of HBV DNA copies in the semen.
J Nanosci Nanotechnol
February 2013
College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, 100049 Beijing, China.
A novel poly(pyrocatechol-3,5-disodiumsulfonate)/multi-walled carbon nanotubes (PPD/MWCNT) composite film modified glassy carbon electrode (GCE) was fabricated and successfully used to simultaneously determine ascorbic acid (AA), dopamine (DA) and uric acid (UA). Owing to the synergistic effects of PPD and MWCNTs, the electrochemical responses of AA, DA and UA at PPD/MWCNTs/GCE were clearly superior to those at bare GCE. Additionally, the PPD/MWCNTs composite film could reduce the potential overlap of AA, DA and UA so as to favor simultaneous determination of the three substances.
View Article and Find Full Text PDFJ Nanosci Nanotechnol
December 2012
Department of Chemistry, Institute of Basic Science, Chonnam National University, 300 Yongbong-Dong, Bukgu, Gwangju 500-757, Korea.
Pd nanoparticle catalysts supported by thiolated graphene oxide (tGO) on a glassy carbon electrode (GCE), and denoted as tGO-Pd/GCE, are used in this study for the electrochemical determination of hydroxylamine and hydrazine. The physicochemical properties of tGO-Pd were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS). They showed strong catalytic activity toward the oxidation of hydroxylamine and hydrazine.
View Article and Find Full Text PDFWei Sheng Yan Jiu
September 2012
Key Laboratory of the Public Health Safety, Ministry of Education, Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China.
Objective: To investigate the effects of (S)-alpha-chlorohydrin (SACH) on rat epididymal sperm motility and hyperactivation and explore the mechanisms of the effects.
Methods: 20 adult male Sprague-Dawley rats were divided randomly into 4 groups and dosed orally with 0, 2.5, 5.
Zhonghua Nan Ke Xue
September 2012
Department of Clinical Laboratory, The First People's Hospital of Guangzhou Affiliated to Guangzhou Medical College, Guangzhou, Guangdong 510180, China.
Objective: To establish a new method for sperm sorting by imitating the physiological process of sperm-cervical mucus interaction on the microfluidic chip.
Methods: We designed a microfluidic chip to imitate the physiological process of natural sperm sorting in the microchannel based on the interaction between sperm and cervical mucus, and obtained motile sperm after the interaction. Meanwhile, we established an integrated real-time sperm detection reservoir on this chip to determine sperm parameters using the computer-assisted sperm analysis system.
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