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Knockout of Pro-Apoptotic BAX and BAK1 Genes in HEK293T Cells Enhances Adeno-Associated Virus (AAV) Production: AAV2 and AAV9.
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Department of Biological Sciences, KAIST, Daejeon, Republic of Korea.
Increasing demand for adeno-associated virus (AAV) used in gene therapy highlights the need to enhance AAV production. When intracellular AAV2 and extracellular AAV9 were produced in HEK293T cells using the triple transfection method, apoptosis occurred during the AAV production. To mitigate apoptosis induced by AAV production, the pro-apoptotic BAX/BAK1 genes were knocked out in HEK293T cells.
View Article and Find Full Text PDFAdjusting for Ascertainment Bias in Meta-Analysis of Penetrance for Cancer Risk.
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Department of Mathematical Sciences, University of Texas at Dallas, Richardson, Texas, USA.
Multi-gene panel testing allows efficient detection of pathogenic variants in cancer susceptibility genes including moderate-risk genes such as ATM and PALB2. A growing number of studies examine the risk of breast cancer (BC) conferred by pathogenic variants of these genes. A meta-analysis combining the reported risk estimates can provide an overall estimate of age-specific risk of developing BC, that is, penetrance for a gene.
View Article and Find Full Text PDFFirst evidence of the effectiveness of a field application of RNAi technology in reducing infestation of the mite Varroa destructor in the western honey bee (Apis mellifera).
Parasit Vectors
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Department of Biology, University of Padova, Padova, Italy.
Background: The mite Varroa destructor is the most serious pest of the western honey bee (Apis mellifera) and a major factor in the global decline of colonies. Traditional control methods, such as chemical pesticides, although quick and temporarily effective, leave residues in hive products, harming bees and operators' health, while promoting pathogen resistance and spread. As a sustainable alternative, RNA interference (RNAi) technology has shown great potential for honey bee pest control in laboratory assays, but evidence of effectiveness in the field has been lacking.
View Article and Find Full Text PDFRibosome pausing in amylase producing Bacillus subtilis during long fermentation.
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Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.
Background: Ribosome pausing slows down translation and can affect protein synthesis. Improving translation efficiency can therefore be of commercial value. In this study, we investigated whether ribosome pausing occurs during production of the α-amylase AmyM by the industrial production organism Bacillus subtilis under repeated batch fermentation conditions.
View Article and Find Full Text PDFGlobal transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer.
Sci Rep
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School of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia University, Morgantown, WV, USA.
The management of micronutrients, such as boron (B) and zinc (Zn), is critical for plant growth and crop yields. One method of rapid intervention crop management to mitigate nutritional deficiency is the foliar supply of B and Zn. Our study investigates the effect of foliar-supplied B and Zn availability on the global transcriptional modulation in soybean (Glycine max).
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