In the present study, complementary spatial modulation of magnetization (CSPAMM) myocardial tagging was extended with an interleaved spiral imaging sequence. The use of a spiral sequence enables the acquisition of grid-tagged images with a tagline distance as low as 4 mm in a single breath-hold. Alternatively, a high temporal resolution of 77 frames per second was obtained with 8-mm grid spacing. Ten healthy adult subjects were studied. With this new approach, high-quality images can be obtained and the tags persist throughout the entire cardiac cycle.
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http://dx.doi.org/10.1002/mrm.10688 | DOI Listing |
ACS Appl Mater Interfaces
January 2025
Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, California 93106, United States.
Controlling cellular shape with micropatterning extracellular matrix (ECM) proteins on hydrogels has been shown to improve the reproducibility of the cell structure, enhancing our ability to collect statistics on single-cell behaviors. Patterning methods have advanced efforts in developing human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) as a promising human model for studies of the heart structure, function, and disease. Patterned single hiPSC-CMs have exhibited phenotypes closer to mature, primary CMs across several metrics, including sarcomere alignment and contractility, area and aspect ratio, and force production.
View Article and Find Full Text PDFSci Adv
December 2024
Department of Applied Physics, Osaka University, Suita, Osaka 565-0871, Japan.
Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman imaging techniques of cryofixed specimens to overcome SNR limitations by enabling long exposure of specimens under highly stabilized low-temperature conditions. The observation of frozen specimens in a cryostat at a constant low temperature immediately after rapid freezing enabled the improvement of SNR and enhanced the spatial and spectral resolution.
View Article and Find Full Text PDFBMC Med Imaging
December 2024
National Engineering Research Center of Advanced Magnetic Resonance Technologies for Diagnosis and Therapy, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Background: Current mainstream cardiovascular magnetic resonance-feature tracking (CMR-FT) methods, including optical flow and pairwise registration, often suffer from the drift effect caused by accumulative tracking errors. Here, we developed a CMR-FT method based on deformable groupwise registration with a locally low-rank (LLR) dissimilarity metric to improve myocardial tracking and strain estimation accuracy.
Methods: The proposed method, Groupwise-LLR, performs feature tracking by iteratively updating the entire displacement field across all cardiac phases to minimize the sum of the patchwise signal ranks of the deformed movie.
Methods Mol Biol
November 2024
Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Proteins are the major and most diverse biomolecules, directing all activities of a cell. For this reason, visualizing protein expression, localization, and dynamics is fundamental in biology. In most cases, protein visualization relies on the overexpression of fluorescently tagged proteins which may not recapitulate endogenous expression pattern and dynamics.
View Article and Find Full Text PDFCurr Res Physiol
August 2024
Department of Physiology, University of Auckland, Auckland, New Zealand.
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