The isothermic adsorption of microbial and animal enzymes on carboxyl and sulpha-cation exchange resins was studied. The adsorption isotherms are curves with a maximum. The adsorption of alpha-amylase was studied in the presence of organic solvents. It was found that organic solvents influenced the isothermic adsorption of alpha-amylase, which is associated with changes in the interactions between protein molecules in solution. The adsorption system was in equilibrium in all the cases.
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Biotechnol Prog
January 2025
Purification Development, Genentech, South San Francisco, California, USA.
Platforms have long been implemented for downstream process development of monoclonal antibodies (mAbs) to streamline development and reduce timelines. These platforms are also increasingly being used for other complex biologics modalities. While development has traditionally been conducted at the lab bench scale in a sequential manner, automated miniaturized and parallelized approaches like RoboColumns and resin plates have also been implemented for chromatographic screening.
View Article and Find Full Text PDFSmall
January 2025
Center of Nanomaterials for Renewable Energy (CNRE), State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, P. R. China.
The traditional perfluorosulfonic acid proton exchange membrane is crucial for proton exchange membrane fuel cells, but its high cost has impeded broader commercialization. In this study, a novel concept of a cost-effective and stable vertically aligned polydopamine-intercalated montmorillonite membrane (VAPMM) is introduced. 2D nanochannels formed within the lamellar structure of polydopamine-coated montmorillonite nanosheets provide a significant stable in-plane proton conductivity of 0.
View Article and Find Full Text PDFFood Chem
January 2025
Laboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, NC Research Campus, 500 Laureate Way, Kannapolis, North Carolina 28081, United States. Electronic address:
Barley (Hordeum vulgare L.; Poaceae), the second most important grain after wheat, contains phenolamides, specifically hordatines and their agmatinated precursors. Hordatines are the unique compounds found in barley, consumption of which is associated with beneficial effects for human health.
View Article and Find Full Text PDFJ Chromatogr A
January 2025
Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów/PL. Electronic address:
Separation of a monoclonal antibody (mAb) from impurities was examined on different cation exchange resins (CEX), including POROS XS, POROS HS, NUVIA S, and NUVIA HRS. Impurities mainly consisted of cell culture-derived mAb fragments, or lysozyme, that mimicked the presence of an adsorbing protein of low molecular weight. The choice between the flowthrough mode and the bind-and-elute mode for the purification was guided by the shape of the adsorption isotherm.
View Article and Find Full Text PDFRSC Adv
January 2025
Electronic Material Research Center, Northwest Institute for Nonferrous Metal Research Xi'an 710016 China.
Potassium is a harmful impurity in the rhenium sinter, which adversely affects its mechanical properties by significantly reducing the density of sintered rhenium. Cationic resin is a promising material for potassium removal. In this study, the strong acid cationic exchange resin C160H was pretreated with an HNO solution to enhance its performance in potassium removal.
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