The diagnosis of drowning is one of the most difficult in forensic pathology. Drowning is death through the aspiration of fluid into the air passages. Signs of immersion only demonstrate submersion of the body for a period of time but are not signs of drowning. The best signs of drowning are froth around the mouth and nostrils and lung distension. Lung histology in drowning victims shows non-specific lesions such as "emphysema aquosum" and alveolar edema. The diatom test for drowning requires a careful protocol to avoid possible contamination. Sample collection (cardiac blood, lung, liver, kidney tissue, bone marrow and brain) should be performed during the first part of the autopsy. Sample preparation requires chemical or enzymatic digestion. The diatom test should be both qualitative and quantitative. Species recovered from the body must correspond to those from the site of drowning and approximately in the same proportions. The diatom test has poor sensitivity and thus is much debated because it is always difficult to rule out possible contamination. Moreover, a negative diatom test cannot rule out drowning as the cause of death. The diagnosis of drowning is based on police investigations, forensic autopsy, microscopic analysis, and biochemical tests, but never solely on pathology findings.
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Int J Legal Med
January 2025
Swedish National Board of Forensic Medicine, Retzius v. 5, 171 65 Stockholm, Stockholm, 171 65, Sweden.
The diagnostic use of the diatom test for drowning has been under investigation for more than a century. Despite continuing research, its true usefulness remains controversial and under debate. Data regarding the extent to which diatoms can penetrate the lungs and other organs of drowning victims are conflicting; similar discrepancies exist as to the presence of diatoms in the organs of living individuals; and as to the occurrence of postmortem (PM) contamination.
View Article and Find Full Text PDFOpen Vet J
November 2024
Department of Clinical Sciences Veterinary Medicine, Veterinary faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Background: There is no specified diagnostic procedure that can help in determining the cause of death and the diagnosis of drowning because the pathohistological signs are almost identical and non-specified.
Aim: Our study aims to recognize and prove diatom appearance in organs from a forensic aspect in Bosnia and Herzegovina, and to examine which is the more specific method in the diagnosis of drowning, the diatom test or the pathohistological finding.
Methods: Rats of the recommended body weight were divided into four groups: G1 ( = 8; mechanism of death-asphyxia; cause of death-suffocation, submerged 1 hour after death); G2 ( = 8: mechanism of death-asphyxia; cause of death-suffocation, immersed 72 hours after death); G3 ( = 8: mechanism of death-asphyxia; cause of death-drowning, autopsy immediately after death), and G4 ( = 8: mechanism of death-asphyxia; cause of death-drowning, post mortem 24 hours after death).
Ecotoxicology
November 2024
Biology Department, College of Science and Engineering, Western Washington University, Bellingham, WA, USA.
Marine diatoms are pervasive in many planktonic and benthic environments and represent an important food source for a wide range of species. Some diatoms produce polyunsaturated aldehydes (PUAs) as defensive toxins. PUA exposure is known to reduce the fecundity of invertebrate grazers like copepods and echinoderm larvae, but little is known about the effects of PUAs on vertebrates.
View Article and Find Full Text PDFInt J Legal Med
November 2024
Université de Paris, CNRS, BABEL, Paris, F-75012, France.
This article evaluates the criteria for diatom testing in forensic investigations, focusing on drowning cases. Diatoms, unicellular algae found in aquatic environments, are critical to the determination of drowning because water containing diatoms is inhaled during submersion. The primary objectives include defining the exact amount and type of tissue to be analyzed, expressed in terms of diatom concentration relative to tissue weight, and detailing the conditions under which water samples are collected to study the diatom flora at the site.
View Article and Find Full Text PDFLeg Med (Tokyo)
November 2024
Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Forensic Medicine, Saitama Medical University, 38 Morohongo, Moroyama, Saitama 350-0495, Japan; Tokyo Medical Examiner's Office, Tokyo Metropolitan Government, 4-21-18, Otsuka, Bunkyo-Ku, Tokyo 112-0012, Japan.
The diatom test is one of the methods used to diagnose drowning in forensic autopsies. Metagenomic diatom analysis may reveal where a drowning occurred. We evaluated whether metagenomic diatom analysis could be used to infer waters, watersheds, and geographic locations using 166 water samples from 64 locations (freshwater: 55; seawater: 9).
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