AI Article Synopsis
- Xenorhabdus nematophila produces protein toxins that effectively kill insect larvae by targeting their hemocytes.
- The study focused on a 17kDa pilin subunit found on the bacterium's surface and in outer membrane vesicles, which was shown to cause harm to the larval hemocytes of Helicoverpa armigera in lab tests.
- The cytotoxic effect of this pilin protein was confirmed, as it caused cell agglutination and the release of lactate dehydrogenase, with its binding to hemocytes being inhibited by specific antiserum.
Article Abstract
Xenorhabdus nematophila is an insect pathogenic bacterium, known to produce protein toxins that kill the larval host. We have described a cytotoxic pilin subunit of X. nematophila, which is expressed on the cell surface and also secreted in the extracellular medium associated with outer membrane vesicles. A 17kDa pilin subunit was isolated and purified from X. nematophila cell surface. The protein showed cytotoxicity to larval hemocytes of Helicoverpa armigera in an in vitro assay, causing agglutination of the cells, and releasing cytoplasmic enzyme lactate dehydrogenase in the medium. The pilin protein was able to bind to the surface of larval hemocytes. The binding and cytotoxicity of the purified 17kDa protein to hemocytes was inhibited by antiserum raised against the pilin protein. The study demonstrates for the first time a cytotoxic structural subunit of pilin from an entomopathogenic bacterium X. nematophila that is excreted in the extracellular medium with outer membrane vesicles.
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| http://dx.doi.org/10.1016/j.bbrc.2003.12.187 | DOI Listing |
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