We describe the detection of the CTX-M-12 beta-lactamase from a clinical isolate of Klebsiella pneumoniae in Colombia. Screening of nosocomial Klebsiella spp. and Escherichia coli isolates from a network of teaching hospitals revealed the presence of CTX-M enzymes in multiple cities. This is the first description of CTX-M in Colombia.
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http://dx.doi.org/10.1128/AAC.48.2.629-631.2004 | DOI Listing |
Heliyon
March 2024
UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador.
The discharge of untreated or partially treated wastewater can have detrimental impacts on the quality of water bodies, posing a significant threat to public health and the environment. In Ecuador, previous research indicates a high prevalence of antimicrobial resistant (AMR) bacteria in surface waters affected by human activities, including irrigation channels. In this study, we analyzed sediment samples collected from an irrigation channel utilized for agricultural purposes in northern Ecuador, using microbiological techniques and whole-genome sequencing (WGS).
View Article and Find Full Text PDFEur J Med Res
January 2023
Department of Microbiology, Faculty of Science, Lagos State University, Lasu Post Office, Ojo, P.O. Box 0001, Lagos, Nigeria.
Background: Resistance to different antimicrobial classes by Salmonella species has generated a global public health concern. The spread of extended-spectrum β-lactamases (ESBLs) bla gene variants is also increasing. This study aimed to investigate the antibiotic resistance and the carriage of bla and bla as well as the quinolone resistance gene (qnrB19) among Salmonella species from hospitalised patients in Lagos, Nigeria.
View Article and Find Full Text PDFMicrobiol Spectr
February 2023
Molecular Diagnostics Research Group, School of Biological and Chemical Sciences, University of Galway, Galway, Ireland.
Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamase (ESBL) enzymes produced by confer resistance to clinically relevant third-generation cephalosporins. CTX-M group 1 variants, CTX-M-1 and CTX-M-15, are the leading ESBL-producing associated with animal and human infection, respectively, and are an increasing antimicrobial resistance (AMR) global health concern. The and genes encoding these variants have an approximate nucleotide sequence similarity of 98.
View Article and Find Full Text PDFInt J Mol Sci
May 2022
Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
Cefotaximase-Munich (CTX-M) extended-spectrum beta-lactamases (ESBLs) are commonly associated with Gram-negative, hospital-acquired infections worldwide. Several beta-lactamase inhibitors, such as clavulanate, are used to inhibit the activity of these enzymes. To understand the mechanism of CTX-M-15 activity, we have determined the crystal structures of CTX-M-15 in complex with two specific classes of beta-lactam compounds, desfuroylceftiofur (DFC) and ampicillin, and an inhibitor, clavulanic acid.
View Article and Find Full Text PDFInfect Genet Evol
December 2021
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP) - Ribeirão Preto, Brazil. Electronic address:
The rapid dissemination of colistin resistance mcr-type genes and extended-spectrum β-lactamase-encoding genes at the human-animal-environment interface has raised concerns worldwide. In this study, we performed a genomic investigation of a multidrug (MDR)- and colistin-resistant Escherichia coli strain recovered from an urban stream strongly affected by pollution and used for recreational purposes in Brazil. E.
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