AI Article Synopsis
- Two water-soluble porphyrins, P1 and P2, were synthesized and analyzed for their photochemical properties and biological effects on human malignant melanoma cells (Me45).
- Both porphyrins showed high UV absorption and low visible light absorption, with P1 being non-toxic up to 50 µg/ml and P2 up to 20 µg/ml when tested in the dark.
- When exposed to red light over 630 nm, both porphyrins effectively inhibited the growth of melanoma cells at these non-toxic concentrations, and most of the cell death observed was necrosis.
Article Abstract
Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 microg/ml (42.14 microM) and P2 at concentrations up to 20 microg/ml (16.86 microM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.
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