Ten known taxoids, paclitaxel, 7-epi-taxol, taxol C, baccatin VI, taxayuntin C, taxuyunnanine C and its analogues (2-5), and yunnanxane (6), and an abietane, taxamairin A, were produced in the callus culture of Taxus cuspidata cultivated on a modified Gamborg's B5 medium in the presence of 0.5 mg/L NAA. After stimulation with 100 microM methyl jasmonate, five more taxoids, cephalomannine, 1beta-dehydroxybaccatin VI, taxinine NN-11 (1), baccatin I, and 2alpha-acetoxytaxusin, and one more abietane, taxamairin C, were found in addition to the above-mentioned compounds. It was also observed that the content of the products increased over three times. Taxinine NN-11 (1) is a new taxane whose structure was assigned as 5alpha,13alpha-diacetoxy-9alpha-cinnamoyloxy-4(20),11-taxadien-10beta-ol by analysis of its spectral data. Taxinine NN-11 (1) exhibited significant MDR reversal activity toward 2780 AD tumor cells. The results of primary screening based on 39 human cancer cell lines suggest that 1 also belongs to a new mechanistic class. Efficient production of 1 was investigated using the callus culture of T. cuspidata.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/np0301083 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Insights on the Mesembryanthemum forsskalii phenotype and study of the effects of several exogenous plant growth regulators via plant tissue culture.
BMC Plant Biol
January 2025
Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Background: Samh (Mesembryanthemum forsskalii, M. cryptanthum) belongs to Aizoaceae family and is found in northern Saudi Arabia, primarily in desert or dry shrubland habitats. M.
View Article and Find Full Text PDFStandardization and consensus in the development and application of bone organoids.
Theranostics
January 2025
Department of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Organoids, self-organized structures derived from stem cells cultured in a specific three-dimensional (3D) microenvironment, have emerged as innovative platforms that closely mimic cellular behavior, tissue architecture, and organ function. Bone organoids, a frontier in organoid research, can replicate the complex structures and functional characteristics of bone tissue. Recent advancements have led to the successful development of bone organoids, including models of callus, woven bone, cartilage, trabecular bone, and bone marrow.
View Article and Find Full Text PDFInsights into total phenolic, flavonoid, and antioxidant activity of callus subculture frequency in rare endemic Ajuga xylorrhiza Kit Tan.
Sci Rep
December 2024
Central Laboratory Application and Research Center, Batman University, Batman, Turkey.
This study assessed the callus morphology, growth index, callus diameter, total antioxidant activity, and total phenolic and flavonoid contents (TPC and TFC) of callus extracts from Ajuga xylorrhiza, a severely endangered plant found in small populations in Türkiye. Calluses obtained from a total of 18 applications formed in culture media supplemented with Kin and 2,4-D at six ratios (A, B, C, D, E, and F) and their subcultures (A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, F1, and F2) were used for examination. Extracts A and D had a higher TFC than the other groups (27.
View Article and Find Full Text PDF<b>Background and Objective:</b> Todolo coffee (<i>Coffea arabica</i> L. var. typica) is the oldest commercially grown coffee in the Toraja region of South Sulawesi and is currently at risk of extinction.
View Article and Find Full Text PDFResearch on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi.
PLoS One
December 2024
Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Central South University of Forestry and Technology, Changsha, China.
Agarwood is a precious resinous heartwood highly valued for its cultural, religious, and medicinal significance. With the increasing market demand, natural agarwood resources are rapidly depleting, making the development of effective artificial induction methods for agarwood highly significant. This study aims to explore the feasibility of using callus tissue to assess the ability of fungi to induce agarwood formation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!