AI Article Synopsis
- Between 1992 and 2000, 4173 rotavirus-positive samples were collected in Hungary, with 2020 (48.4%) analyzed for G serotype using immunoassay and PCR methods.
- The analysis identified various G serotypes, with G1 being the most common at 62%, while 11.4% of samples could not be typed.
- Both common and uncommon rotavirus strains were found, with the newly identified P[9],G6 and P[8],G9 strains potentially representing significant additions to the known serotypes in Hungary.
Article Abstract
Between 1992 and 2000, a total of 4173 rotavirus-positive samples were collected from two areas of Hungary. Of these, 2020 specimens (48.4%) were analyzed for G serotype, using monoclonal antibody-based immunoassay and reverse transcription-PCR. By the two methods, 1789 samples were specified as G1 (62%), G2 (12.2%), G3 (1.4%), G4 (6.4%), G6 (1.0%), G9 (2.9%), or mixed infection (2.6%), and the remaining 231 (11.4%) could not be G typed. The linkage between G and P type, subgroup specificity, and RNA profile was investigated with a sample subset. Among these specimens, we identified both the four globally common strains (P[8],G1 subgroup II (sgII); P[4],G2 sgI; P[8],G3 sgII; and P[8],G4 sgII) and six uncommon strains (P[6],G4 sgII; P[9],G3 sgI; P[9],G6 sgI; P[14],G6 sgI; P[8],G9 sgII; and P[8],G9 sgI). All strains with P[8], P[6], P[9], and P[14] specificities had a long electropherotype, whereas most of those carrying a P[4] specificity were associated with a short electropherotype. Although once considered to be rare, P[9],G6 and P[8],G9 rotavirus strains represent potentially important new serotypes in Hungary.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC321674 | PMC |
| http://dx.doi.org/10.1128/JCM.42.1.393-397.2004 | DOI Listing |
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