Techniques for studying the oxygen-sensitive transcription factor FNR from Escherichia coli.

Methods Enzymol

Department of Biomolecular Chemistry, University of Wisconsin, 574 Medical Science Center, 1300 University Avenue, Madison, Wisconsin 53706, USA.

Published: February 2004

A large variety of techniques can be adapted for use with oxygen-sensitive samples. The growth of cells and in vivo analyses, as well as protein purification and in vitro assays, can be executed either by performing necessary steps in anaerobic environments (ranging from simple closed containers to the anaerobic chamber) or by circumventing the need for anaerobiosis with the use of oxygen-resistant protein variants.

Download full-text PDF

Source
http://dx.doi.org/10.1016/S0076-6879(03)70027-5DOI Listing

Publication Analysis

Top Keywords

techniques studying
4
studying oxygen-sensitive
4
oxygen-sensitive transcription
4
transcription factor
4
factor fnr
4
fnr escherichia
4
escherichia coli
4
coli large
4
large variety
4
variety techniques
4

Similar Publications

The U.S. Developmental Origins of Health and Disease (DOHaD) meeting is an annual conference of primarily U.

View Article and Find Full Text PDF

Background: Mental health remains among the top 10 leading causes of disease burden globally, and there is a significant treatment gap due to limited resources, stigma, limited accessibility, and low perceived need for treatment. Problem Management Plus, a World Health Organization-endorsed brief psychological intervention for mental health disorders, has been shown to be effective and cost-effective in various countries globally but faces implementation challenges, such as quality control in training, supervision, and delivery. While digital technologies to foster mental health care have the potential to close treatment gaps and address the issues of quality control, their development requires context-specific, interdisciplinary, and participatory approaches to enhance impact and acceptance.

View Article and Find Full Text PDF

It has been well accumulated that G-quadruplex (G4-DNA) has great anticancer relevance, and various heterocyclic moieties have been synthesized and examined as potent G4-DNA binders with promising anticancer activity. Here, we have synthesized a series of naphthalimide-triazole-coumarin conjugates by substituting various amines and further examine their anticancer activity against 60 human cancer cell lines at 10 μM. One and five dose concentration results reveal low values of MG-MID GI for compounds including (3.

View Article and Find Full Text PDF

Background: Multifrequency MR elastography (mMRE) enables noninvasive quantification of renal stiffness in patients with chronic kidney disease (CKD). Manual segmentation of the kidneys on mMRE is time-consuming and prone to increased interobserver variability.

Purpose: To evaluate the performance of mMRE combined with automatic segmentation in assessing CKD severity.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!