AI Article Synopsis
- Developed two molecular techniques to quickly and specifically detect bacterial pathogens (E. coli, P. aeruginosa, and Salmonella spp.) in water samples.
- Optimized a multiplex-PCR assay using specific primer pairs for each pathogen, allowing for simultaneous detection.
- Achieved results indicating that the M-PCR assay significantly outperforms traditional methods, providing accurate results within 5 hours from sampling.
Article Abstract
In this work we developed and optimized two molecular-based approaches to monitor rapidly, sensitively and specifically bacterial pathogens from three different genera, Escherichia coli, Pseudomonas aeruginosa, and Salmonella spp., directly in waters. To achieve this aim, firstly a multiplex-PCR assay (M-PCR) was optimized using a primer pair specific for each pathogen. Secondly, as a molecular confirmatory test after isolation of the pathogens by classical microbiological methods, PCR-RFLP of their amplified 16S rDNA genes was performed. It was observed from the results that the developed M-PCR assay has significant impact on the ability to detect sensitively, rapidly and specifically the three pathogens directly in water within a short time (5 h from sampling to obtain final results), therefore it represents a considerable advancement over other known more time-consuming and less-sensitive methods for identification and characterization of these kinds of pathogens.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/b307476e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Effect of Single Nucleotide Polymorphisms on Clinical Phenotypes of Sabahan Transfusion-Dependent β-Thalassemia Patients with Homozygous Filipino β-Deletion.
Hemoglobin
January 2025
Department of Biomedical and Science Therapeutic, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.
Sabah has the highest prevalence of β-thalassemia in Malaysia, with the Filipino β-deletion as the predominant mutation. Patients with the homozygous Filipino β-deletion exhibit phenotypic heterogeneity due to various genetic modifiers, yet the effects of these modifiers on the clinical phenotype remain poorly understood. This study investigated the effects of the coinheritance of α-thalassemia, I-γ rs7482144, rs766432, and 5'HS4 rs16912979 polymorphisms on the clinical phenotype of homozygous Filipino β-deletion patients in Sabah.
View Article and Find Full Text PDFPresence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study.
J Bras Nefrol
January 2025
Universidade Federal Fluminense, Faculdade de Medicina, Departamento de Medicina Clínica, Niterói, RJ, Brazil.
Introduction: The World Health Organization (WHO) points out that infection by enteroparasites can affect ~3.5 billion people around the world. Hemodialysis (HD) patients may be more susceptible to infections by opportunistic pathogens due to impaired immune function.
View Article and Find Full Text PDFTrend in serological and molecular diagnostic methods for Toxoplasma gondii infection.
Eur J Med Res
October 2024
Health Park Co., Ltd, Seoul, 02447, Republic of Korea.
Background: Toxoplasma gondii, an intracellular parasite, is a significant cause of zoonotic disease, with an estimated one-third of the world's human population believed to be infected. T. gondii is transmitted to humans through the consumption of contaminated water, soil, vegetables, fruits, shellfish or undercooked meat, and can also be passed from human to human through vertical transmission, transplants and blood transfusion.
View Article and Find Full Text PDFAssessment of the contribution of and genes in the pathogenesis of celiac disease.
J Genet
October 2024
Immunogenomics Laboratory, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Bathinda 151 401, India.
Vitamin-D deficiency (VDD) is a global health concern. It is known to play a critical role in the immunomodulation, and thus, its metabolism could be investigated to unravel its contribution in common immune-mediated diseases, e.g.
View Article and Find Full Text PDFA rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates.
Diagn Microbiol Infect Dis
December 2024
Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India. Electronic address:
Article Synopsis
- Giardia duodenalis is a major cause of diarrhea in low-resource areas, necessitating quick and affordable detection methods for studies and clinical use.
- A new multiplex PCR-RFLP technique targeting the tpi gene was developed and successfully identified Giardia in 6.33% of clinical samples while classifying them into different genetic groups.
- The method showed high reliability with sensitivity at 94.2%, specificity at 100%, and accuracy at 97.1%, making it a promising tool for diagnosing giardiasis effectively and economically.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!