Objective: The purpose of this study was to determine the effect of combined CO2 and Er:YAG laser irradiation on normal (NF) and keloid (KF) facial dermal fibroblast production of TGF-beta1 and bFGF.
Background Data: Keloids produce excess collagen. TGF-beta1 is integral to the growth and stimulation of fibroblasts and collagen; bFGF inhibits collagen synthesis. TGF-beta1 and bFGF production influence wound healing and may be manipulated by laser irradiation.
Materials And Methods: Human normal fibroblasts (NF) and keloid fibroblasts (KF) (2 x 10(4) cells/mL in serum-free media) were exposed to 1.7 J/pulse Er:YAG laser energy and CO2 delivered at either 3 or 5 W and at a duty cycle of 25%, 50%, or 100%. TGF-beta1 and bFGF were assayed using a quantitative ELISA.
Results: KF demonstrated a statistically significant mean population doubling time (PDT) when compared with NF (p=0.01). Irradiated KF and NF had longer PDTs than controls. All NF, excluding one irradiated group, and the three KF treated with 3 W secreted more bFGF than controls. Irradiated KF secreted less TGF-beta1 than controls. Significance was reached with the two groups exposed to 3 W at a duty cycle of 25% and 50% (p=0.04 and 0.05, respectively). All irradiated NF secreted less TGF-beta1 than controls.
Conclusion: The combined CO2 and Er:YAG laser increased the release of bFGF, which has been shown to promote tightly organized collagen bundles, and decreased the concentration of TGF-beta1, which has also been shown to promote fibrosis formation. This laser may have a future role in keloid treatment, as well as normal facial scar prevention.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/104454703322650130 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhanced vertical bone augmentation in rat parietal bone using hydroxyapatite-coated polytetrafluoroethylene domes with Erbium Yttrium Aluminum Garnet pulsed laser deposition.
J Prosthodont Res
January 2025
Department of Orthodontics, Osaka Dental University, Hirakata, Japan.
Purpose: To perform vertical bone augmentation on rat parietal bone by coating the inner surface of dense polytetrafluoroethylene (d-PTFE) domes with hydroxyapatite (HA) using Erbium Yttrium Aluminum Garnet (Er:YAG) pulsed laser deposition in a rat model.
Methods: The d-PTFE plate surface, α-tricalcium phosphate (α-TCP) coating, and HA coating were measured using scanning electron microscopy and X-ray diffraction to confirm the replacement of α-TCP with HA via high-pressure steam sterilization. The dome was glued to the center of the rat parietal bone and closed with periosteal and epithelial sutures.
Effect of different surface conditioning methods and low pH solutions on the shear bond strength of orthodontic brackets to newly introduced CAD/CAM materials.
Heliyon
November 2024
Department of Orthodontics, Faculty of Dentistry, University of Health Sciences, Turkey.
Aim: To evaluate the shear bond strength (SBS) of ceramic and metallic orthodontic brackets bonded to lithium disilicate ceramics or hybrid ceramics and subjected to different surface conditioning treatments.
Materials And Methods: In total, 300 specimens were fabricated from GC LiSi (lithium disilicate) and GC Cerasmart (hybrid) ceramic blocks. The specimens were divided into four groups according to the following surface treatments: hydrofluoric acid (HF); sandblasting with 50 μm aluminum oxide; Monobond Etch and Prime; and erbium-doped yttrium aluminum garnet (Er-YAG) laser.
Dentinal Tubule Occluding Efficacy Of Er: YAG Laser And Universal Adhesive Loaded With Nano-Carbonated Apatite.
Braz Dent J
July 2024
Conservative Dentistry Department, Faculty of Dentistry, Cairo University, Egypt. Postal Code: 11553.
ER:YAG laser and experimental resin-based dental adhesive loaded with functionalized carbonated apatite filler were used in this study to evaluate the dentin interaction in terms of penetration and occlusion of the dentinal tubules aiding in the control of dentin hypersensitivity (DH). Spheroidal Carbonated apatite nanoparticles (N-CAP), with an average size of 20±5 nm diameter, were synthesized, characterized, and incorporated in a universal adhesive "All Bond Universal, Bisco, USA", in (2% weight) concentration. Er:YAG laser "Lightwalker, FOTONA, EU" was adjusted to an energy output of 40mJ/ pulse and pulse repetition of 10 Hz for 10 seconds.
View Article and Find Full Text PDFRoot surface roughness evaluation following application of different periodontal instruments and Er:YAG laser: A profilometry and SEM study.
Lasers Med Sci
April 2024
Department of Periodontology, Hamidiye Dental Faculty, University of Health Sciences, Istanbul, Turkey.
Aim: The aim of the present study was to evaluate the efficacy of 30-angled Er:YAG laser tip and different periodontal instruments on root surface roughness and morphology in vitro.
Methods: Eighteen bovine teeth root without carious lesion were decoronated from the cementoenamel junction and seperated longitidunally. A total of 36 obtained blocks were mounted in resin blocks and polished with silicon carbide papers under water irrigation.
Use of Phototherapy and Er-YAG Laser in the Management of Mandible Osteoradionecrosis: A Case Report.
J Lasers Med Sci
November 2023
School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil.
Article Synopsis
- * Following her diagnosis, she had multiple dental extractions that led to bone exposure and complications, which were treated with photobiomodulation therapy and antimicrobial photodynamic therapy, resulting in improved clinical conditions.
- * After removing necrotic bone with a specialized laser, the patient successfully received dental prosthetics and is currently being monitored, showing no signs of recurrence, indicating potential effectiveness of laser treatments for ORN.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!