Colibacillosis caused by Escherichia coli infections account for significant morbidity and mortality in the poultry industry. Yet, despite the importance of colibacillosis, much about the virulence mechanisms employed by avian E. coli remains unknown. In recent years several genes have been linked to avian E. coli virulence, many of which reside on a large transmissible plasmid. In the present study, a multiplex polymerase chain reaction (PCR) protocol to detect the presence of four of these genes is described. Such a protocol may supplement current diagnostic schemes and provide a rapid means of characterizing the E. coli causing disease in poultry. The targets of this procedure included iss, the increased serum survival gene; tsh, the temperature sensitive hemagglutinin gene; cvi, the ColV immunity gene; and iucC, a gene of the aerobactin operon. Organisms, known for their possession or lack of these genes, were used as a source of the template DNA to develop the multiplex PCR protocol. Identity of the amplicons was confirmed by size, DNA:DNA hybridization with specific gene probes, and DNA sequencing. When the multiplex PCR protocol was used to characterize 10 E. coli isolates incriminated in avian colibacillosis and 10 from the feces of apparently healthy birds, nine of the isolates from apparently healthy birds contained no more than one gene, while the 10th contained all four. Also, eight of the isolates incriminated in colibacillosis contained three or more genes, while the remaining two contained two of the target genes. Interestingly, the isolates of sick birds containing only two of the targeted genes killed the least number of embryos,and the isolate of healthy birds that contained all the genes killed the most embryos amongthis group. These genes were not found among the non-E. coli isolates tested, demonstrating the procedure's specificity for E. coli. Overall, these results suggest that this protocol might be useful in characterization and study of avian E. coli.
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http://dx.doi.org/10.1637/7030 | DOI Listing |
Lett Appl Microbiol
January 2025
Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, 37, K.B. Sarani, Belgachia, Kolkata, West Bengal, India.
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View Article and Find Full Text PDFBMC Vet Res
January 2025
Faculty of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Background: Lower urinary tract disease is a common clinical condition in dogs, usually presenting with dysuria, pollakiuria and haematuria. Diabetes mellitus is a predisposing factor for urinary tract infection in both humans and dogs and does not necessarily present with clinical signs. In this case report, we describe for the first time a case of cystitis glandularis in a dog with diabetes mellitus, associated with Escherichia coli urinary tract infection.
View Article and Find Full Text PDFJ Infect Chemother
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Department of Urology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Introduction: Febrile urinary tract infections are major complications of radical cystectomy; however, their characteristics after robot-assisted radical cystectomy remain unclear. Thus, we investigated the rate, severity, pathogens, and risk factors of febrile urinary tract infections after robot-assisted radical cystectomy.
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Poult Sci
January 2025
Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, PR China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, PR China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, PR China. Electronic address:
Escherichia coli (E. coli) is a significant pathogen responsible for intestinal infections and foodborne diseases. The rise of antibiotic resistance poses a significant challenge to global public health.
View Article and Find Full Text PDFInt J Food Microbiol
January 2025
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58, Rinkuourai-kita, Izumisano, Osaka 598-8531, Japan; Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan; Asian Health Science Research Institute, Osaka Metropolitan University, Osaka, Japan; Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University, 1-58, Rinkuourai-kita, Izumisano, Osaka 598-8531, Japan. Electronic address:
Since cefixime and tellurite are known to inhibit most bacteria belonging to Enterobacterales, we found that addition of tellurite inhibited E. albertii growth in Luria Bertani broth but not in tryptic soy broth (TSB), and addition of phosphate and soy peptone enhanced E. albertii growth in TSB in presence of tellurite.
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