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Flow Cytometry and Cell Cycle Analysis: An Overview.
Methods Mol Biol
September 2022
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada.
Cell cycle analysis is one of the earliest applications in flow cytometry and continues to be highly used to this day. Since the first reported method of Feulgen-DNA staining, cell cycle analysis has continued to grow and mature. With the recent advances in DNA dyes, understanding of additional cell cycle phase markers, and new technologies, cell cycle analysis continues to be a dynamic field within the flow cytometry community.
View Article and Find Full Text PDFCryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization.
Cytogenet Genome Res
June 2012
Dipartimento di Biologia Cellulare e Ambientale, Sezione di Biologia Cellulare e Molecolare, Università di Perugia, Italia.
Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from Triticum aestivum cv. 'Chinese Spring' (CS) × Dasypyrum villosum(Dv) intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from Dv were apparently added to their complement.
View Article and Find Full Text PDFCytochemistry and C-values: the less-well-known world of nuclear DNA amounts.
Ann Bot
April 2008
Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University of Vienna, Rennweg 14, A 1030 Vienna, Austria.
Background: In the plant sciences there are two widely applied technologies for measuring nuclear DNA content: Feulgen absorbance cytophotometry and flow cytometry (FCM). While FCM is, with good reasons, increasingly popular among plant scientists, absorbance-cytophotometric techniques lose ground. This results in a narrowing of the methodological repertoire, which is neither desirable nor beneficial.
View Article and Find Full Text PDFGenome sizes of cranes (Aves: Gruiformes).
J Morphol
December 2006
Department of Anatomy and Cell Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614-0582, USA.
The DNA content of blood cell nuclei of 15 species of cranes was determined by Feulgen-DNA cytophotometry. Genome sizes agree with values reported elsewhere for several crane species analyzed by flow cytometry. Males have more DNA per cell than females in several species.
View Article and Find Full Text PDFNucleus image properties assessed by video image analysis in mouse hepatocytes under a short lysis for extended chromatin fiber formation.
Cytometry A
November 2006
Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
Background: How much DNA remains in mouse hepatocyte nuclei after extended chromatin fiber (ECF) formation or whether this content varies within the nuclear population is not known. This information could be relevant to understanding chromatin extensibility as related to chromatin organization, possibly associated with variable nuclear activities in hepatocytes.
Methods: A protocol for ECF formation under the gravity action, image analysis of Feulgen-stained unfixed mouse hepatocyte remnants, and DAPI fluorescence were used.
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