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DNA-based identification of Brassica vegetable species for the juice industry. | LitMetric

DNA-based identification of Brassica vegetable species for the juice industry.

J Nutr Sci Vitaminol (Tokyo)

Central Research Institute, Q'sai Co. Ltd., Munakata, Fukuoka 811-3422, Japan.

Published: October 2003

AI Article Synopsis

  • Kale is a nutrient-rich cruciferous vegetable popular in the juice industry, making quality control essential to avoid mixing it with similar species like cabbage.
  • Researchers developed a DNA-based method to identify kale and cabbage, using random amplified polymorphic DNA (RAPD) to detect specific DNA markers unique to cabbage.
  • By designing specific primers from these markers, the team successfully distinguished multiple cabbage varieties from different kale samples, ensuring higher quality in kale juice products.

Article Abstract

Since kale (Brassica oleracea var. acephala), a cruciferous vegetable with a high level of vitamins and functional compounds beneficial to health and wellness, has become widely used in the juice industry, a precise method for quality control of vegetable species is necessary. We describe here a DNA-based identification method to distinguish kale from cabbage (Brassica oleracea var. capitata), a closely related species, which can be inadvertently mixed with kale during the manufacturing process. Using genomic DNA from these vegetables and combinatory sets of nucleotide primers, we screened for random amplified polymorphic DNA (RAPD) fragments and found three cabbage-specific fragments. These RAPD fragments, with lengths of 1.4, 0.5, and 1.5 kb, were purified, subcloned, and sequenced. Based on sequence-tagged sites (STS), we designed sets of primers to detect cabbage-specific identification (CAI) DNA markers. Utilizing the CAI markers, we successfully distinguished more than 10 different local cabbage accessions from 20 kale accessions, and identified kale juices experimentally spiked with different amounts of cabbage.

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Source
http://dx.doi.org/10.3177/jnsv.49.357DOI Listing

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