Peroxynitrite (ONOO-) has been recently known to act as a potent cytotoxin during pathogenesis of various diseases. This study aimed to investigate the possible effect of ONOO- on the cremaster muscle arteriolar reactivity in response to noradrenaline and subsequently determined whether membrane hyperpolarization and potassium channel activation were involved in ONOO(-)-induced alteration of arteriolar reactivity. The results demonstrated that 1) ONOO- could decrease arteriolar reactivity in a time- and concentration-dependent manner with no significant alteration of arteriolar diameter; 2) Superfusion with 20 microM ONOO- over 40 minutes showed slight but not significant influence on the resting potential (Em) of arteriolar smooth muscle cells (ASMCs). However, ASMCs subjected to 50 or 100 microM ONOO- administration were significantly hyperpolarized. As control, treatment with 50 microM decomposed ONOO- or Kreb's solution had little effect on the Em of ASMCs; 3) ONOO(-)-induced arteriolar hyporeactivity could be greatly reversed by co-administration of KCl and partially by TEA. The above results indicated that membrane hyperpolarization and potassium channel activation were preferentially responsible for the reduction of cremaster muscle arteriolar reactivity after exposure to ONOO-.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.lfs.2003.07.039 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Melatonin improves adverse vascular remodelling and redox homeostasis in monocrotaline-induced pulmonary arterial hypertension.
Arch Physiol Biochem
January 2025
Laboratório de Fisiologia Cardiovascular, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
This study explored the effects of melatonin on cardiac and vascular function, and redox homeostasis in model PAH. Male Wistar rats were divided into: control (CTR), monocrotaline [MCT (60 mg/kg, single dose i.p)], monocrotaline + sildenafil [MCT + SIL (50 mg/kg/day)], and monocrotaline + melatonin [MCT + MEL (10 mg/kg/day)].
View Article and Find Full Text PDFEffects of long-term almond consumption on markers for vascular function and cardiometabolic risk in men and women with prediabetes: results of a randomized, controlled cross-over trial.
Eur J Nutr
November 2024
Department of Nutrition and Movement Sciences, Research Institute of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.
Article Synopsis
- This study aimed to explore the long-term effects of eating almonds on vascular health and blood pressure in people with obesity and prediabetes.
- Thirty-four participants went through a trial where they consumed 50g of almonds daily for five months, with measurements taken on vascular function and inflammatory markers before and after the intervention.
- Results showed that while almond consumption did not significantly change arterial stiffness or various serum markers, it did lower overall blood pressure and its variability, suggesting potential benefits for managing blood pressure without affecting arterial health.
Regulation of Skeletal Muscle Resistance Arteriolar Tone: Temporal Variability in Vascular Responses.
J Vasc Res
December 2024
Departments of Medical Biophysics, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.
Introduction: A full understanding of the integration of the mechanisms of vascular tone regulation requires an interrogation of the temporal behavior of arterioles across vasoactive challenges. Building on previous work, the purpose of the present study was to start to interrogate the temporal nature of arteriolar tone regulation with physiological stimuli.
Methods: We determined the response rate of ex vivo proximal and in situ distal resistance arterioles when challenged by one-, two-, and three-parameter combinations of five major physiological stimuli (norepinephrine, intravascular pressure, oxygen, adenosine [metabolism], and intralumenal flow).
Impaired Peripheral Vascular Function Following Ischemic Stroke in Mice: Potential Insights into Blood Pressure Variations in the Post-Stroke Patient.
Pathophysiology
September 2024
Molecular & Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA 71103, USA.
High systolic blood pressure and increased blood pressure variability after the onset of ischemic stroke are associated with poor clinical outcomes. One of the key determinants of blood pressure is arteriolar size, determined by vascular smooth muscle tone and vasodilatory and vasoconstrictor substances that are released by the endothelium. The aim of this study is to outline alterations in vasomotor function in isolated peripheral arteries following ischemic stroke.
View Article and Find Full Text PDFComparison of Adiposomal Lipids between Obese and Non-Obese Individuals.
Metabolites
August 2024
Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, College of Medicine, University of Illinois Chicago, Chicago, IL 60612, USA.
Article Synopsis
- - Our study found that extracellular vesicles (adiposomes) from fat tissue differ between obese and lean individuals, particularly in their lipid content, which might influence cardiovascular health and metabolic conditions.
- - Adiposomes from obese people had more ceramides and fewer lysophosphatidylcholine/phosphatidylcholine lipids compared to those from lean individuals, indicating a significant difference in lipid profiles.
- - We observed strong connections between these lipid profiles and various health factors like body mass index (BMI), insulin resistance, and blood vessel function, suggesting that altered lipid cargo in adiposomes could affect cardiometabolic health in obesity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!