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Diffusion ordered spectroscopy as a complement to size exclusion chromatography in oligosaccharide analysis. | LitMetric

AI Article Synopsis

  • Researchers studied N-acetyl-chitooligosaccharides using a nuclear magnetic resonance technique called diffusion ordered spectroscopy (DOSY), focusing on their diffusion coefficients and molecular weights.
  • They found a strong linear relationship between the log of diffusion coefficients and molecular weight, allowing for accurate estimates of synthetic chitin derivatives.
  • The study also explored interactions between a chitin-binding protein (hevein) and the oligosaccharide (GlcNAc)6, revealing different binding complexes based on ligand concentration, and highlighted DOSY's advantages over traditional methods in carbohydrate analysis.

Article Abstract

A series of N-acetyl-chitooligosaccharides (GlcNAc)(1-6) have been studied by a nuclear magnetic resonance (NMR) method, diffusion ordered spectroscopy (DOSY). DOSY has also been applied to two additional synthetic related oligosaccharides [GlcNH(2)-(GlcNAc)(4) and GlcNH(2)-(GlcNAc)(2)-GlcNAcSO(3)Na]. A plot of the log of the determined diffusion coefficients (logD) of (GlcNAc)(n) versus the log of molecular weight was linear (6 points, R(2) = 0.995). The molecular weights of the two synthetic chitin derivatives could be estimated to within 10% error. The processed NMR data of all the chitooligosaccharides was also plotted in a polyacrylamide gel-like format to aid visual interpretation. Moreover, the logD value of the NMR signal resonances of a chitin-binding protein (hevein) changed as a function of a given titrated ligand, (GlcNAc)(6). Evidence for a 2:1 hevein:(GlcNAc)(6) complex is detected by DOSY at high hevein:(GlcNAc)(6) ratios. This data is consistent with published analytical ultracentrifugation and isothermal titration calorimetry data. A 1:1 complex is preferred at higher ligand concentrations. DOSY can complement size exclusion chromatography in carbohydrate research with the advantage that oligosaccharides are more readily detected by NMR.

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Source
http://dx.doi.org/10.1093/glycob/cwh037DOI Listing

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