AI Article Synopsis
- The study focuses on the formation of structurally isomeric complexes between homopyrimidine bis-PNAs and different forms of DNA (single- and double-stranded).
- Isomers S1, S2, and S3 are identified based on their mobility in polyacrylamide gel electrophoresis, with S3 being the most stable and forming exclusively on double-stranded DNA.
- The research also finds that the stability of isomer S1 significantly increases when an excess of complementary single-stranded oligonucleotide is present, and proposes that the S1 structure consists of two bis-PNA/DNA triplexes.
Article Abstract
Structurally isomeric complexes formed between homopyrimidine bis-PNAs (T(2)JT(2)JT(4)-linker-T(4)CT(2)CT(2)) and single- and double-stranded DNA targets were investigated. These complexes are triplexes designated S1, S2 and S3 in order of increased mobility by polyacrylamide gel electrophoresis. It is shown that the S3 isomer is formed only on double-stranded DNA and possesses highest stability. Isomers S2 and S1 are formed upon binding of bis-PNA to double-stranded as well as to single-stranded DNA. It was found that the stability of the isomer S1 increases dramatically in the presence of excess single-stranded oligonucleotide complementary to the bis-PNA. The structure of the stabilized S1 isomer is proposed to consist of two bis-PNA/DNA triplexes. The relationship between the yield of the isomer S1 formed on single-stranded DNA and the bis-PNA concentration was investigated and a kinetic model of the formation of S1 is presented.
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| http://dx.doi.org/10.1080/07391102.2004.10506944 | DOI Listing |
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