AI Article Synopsis
- Polyclonal antibodies against the insecticide fenthion were previously developed, leading to the creation of an indirect competitive ELISA for detecting this pesticide.
- The current study established direct competitive ELISAs for fenthion using microtiter plates and dipstick formats, achieving an IC(50) of 1.2 microg/L and 15 microg/L, respectively.
- The dipstick format provided quick visual detection and demonstrated recovery rates of 87-116% for fenthion in spiked vegetable samples without any prior processing.
Article Abstract
In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA showed an IC(50) value of 1.2 microg/L with a detection limit of 0.1 microg/L. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides. The use of the dipstick format using Immunodyne as a support membrane allowed the quick visual detection of fenthion in concentrations >10 microg/L. The IC(50) value of the dipstick format using reflectance detection was 15 microg/L with a detection limit of 0.5 microg/L. The recoveries of fenthion from spiked vegetable samples using the two formats without any prior enrichment or cleanup steps were 87-116%.
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| http://dx.doi.org/10.1021/jf0346915 | DOI Listing |
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