In classical chromatolysis, Nissl bodies are initially lost centrally with subsequent progression to the periphery of the neuron. Peripheral chromatolysis has the opposite pattern; it is less common and more difficult to produce. We describe a new method for producing peripheral chromatolysis in neurons of trigeminal ganglia and dorsal root ganglia that requires only injection of large doses of lithium chloride (LiCl) for two, three or four consecutive daily doses. This method may be useful for elucidating the intraneuronal mechanisms that control the location and structure of the Nissl bodies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jneumeth.2003.07.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
miR-30c-5p Gain and Loss of Function Modulate Sciatic Nerve Injury-Induced Nucleolar Stress Response in Dorsal Root Ganglia Neurons.
Int J Mol Sci
October 2024
Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad de Cantabria, 39011 Santander, Spain.
Article Synopsis
- * Previous research found that increasing miR-30c levels can worsen neuropathic pain, while reducing it can prevent pain onset and reverse allodynia.
- * This study focused on the role of miR-30c-5p, revealing that it worsens neuron damage and stress in the DRG after nerve injury, suggesting that targeting miR-30c-5p could offer a new way to treat neuropathic pain.
Bilateral stringhalt associated with Hypochaeris radicata in Uruguayan horses.
Toxicon
July 2024
Plataforma de Investigación en Salud Animal (PSA), Instituto Nacional de Investigación Agropecuaria (INIA), Estación Exprerimental Tacuarembó, Uruguay.
We reported thirteen cases of bilateral stringhalt associated with Hypochaeris radicata that occurred in horses in Uruguay during a severe drought in the summer of 2023. All horses were affected chronically and progressively by bilateral hyperflexion of hindlimbs. In two severely affected horses, the main histological lesions included neuronal chromatolysis and axonal spheroids in the ventral gray horn in the lumbar and sacral spinal cord and axonal degeneration and digestion chambers in ventral roots fibers and long peripheral nerves.
View Article and Find Full Text PDFThe assessment of neuronal plasticity following sciatic nerve injuries in rats using electron microscopy and stereological methods.
J Chem Neuroanat
March 2024
School of Life Science and Bioengineering, Nelson Mandela-African Institution of Science and Technology, Arusha, Tanzania; Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkiye. Electronic address:
The transmission of signals to the cell body from injured axons induces significant alterations in primary sensory neurons located in the ganglion tissue, the site of the perikaryon of the affected nerve fibers. Disruption of the continuity between the proximal and distal ends leads to substantial adaptability in ganglion cells and induces macrophage-like activity in the satellite cells. Research findings have demonstrated the plasticity of satellite cells following injury.
View Article and Find Full Text PDFStanding gustatory papillae biopsy procedure for antemortem diagnosis of equine grass sickness.
J Am Vet Med Assoc
February 2024
1Ecole Nationale Vétérinaire d'Alfort, CHUV-Equidés, Maisons-Alfort, France.
Objective: Diagnosing equine grass sickness (EGS) requires histopathological evidence of chromatolysis and/or neuronal loss in peripheral autonomic ganglia. Previous investigators performed postmortem biopsies of gustatory papillae located on the tongue and found chromatolytic subgemmal neurons in all 13 EGS horses. The present study aimed to design a standardized lingual biopsy sampling method through a transbuccal approach in healthy standing horses and assess the quality of the obtained samples, to allow antemortem diagnosis of EGS in clinical cases.
View Article and Find Full Text PDFThe histomorphological and stereological assessment of rat dorsal root ganglion tissues after various types of sciatic nerve injury.
Histochem Cell Biol
February 2024
Faculty of Medicine, Department of Histology and Embryology, Ondokuz Mayıs University, Samsun, Türkiye.
Peripheral nerve injuries lead to significant changes in the dorsal root ganglia, where the cell bodies of the damaged axons are located. The sensory neurons and the surrounding satellite cells rearrange the composition of the intracellular organelles to enhance their plasticity for adaptation to changing conditions and response to injury. Meanwhile, satellite cells acquire phagocytic properties and work with macrophages to eliminate degenerated neurons.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!