Crystallization of the pectate lyase PelI from Erwinia chrysanthemi and SAD phasing of a gold derivative.

Acta Crystallogr D Biol Crystallogr

Composante INSA de l'Unité de Microbiologie et Génétique, UMR 5122 CNRS-UCBL-INSA, Bâtiment A. Lwoff, 10 Rue R. Dubois, 69622 Villeurbanne, France.

Published: January 2004

The pectate lyase PelI is involved in the degradation of plant tissues by the phytopathogenic bacterium Erwinia chrysanthemi. It has been crystallized from a solution containing PEG 550 in the space group P2(1), with unit-cell parameters a = 61.6, b = 70.7, c = 73.4 A, beta = 112.8 degrees. Crystals diffract to 1.45 A using synchrotron radiation. SAD phases have been computed from a gold-derivative crystal at the wavelength of maximum absorption (L(III) edge).

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http://dx.doi.org/10.1107/s0907444903026222DOI Listing

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