[Characterization of novel alleles and new mutation sites of porcine MHC class II DQA].

The primers, DQAp161 and DQAp443, were designed based on the homologous region of SLA-DQA cDNA sequences and HLA-DQA genomic sequences. The 731 bp fragment of SLA-DQA including completed intron 2, the near completed exon 2 and partial exon 3 was obtained by PCR. The nucleotide sequences of the fragment of SLA-DQA were obtained with cloning and direct sequencing. Both nucleotide sequences of exon 2 and amino acid sequences of alpha 1 domain were analyzed in a pedigree. The sequence data were compared with all sequences of SLA-DQA exon 2 in GenBank. Two novel alleles, DQA-SLT 26 and DQA-TC 21-1, were found according to the above analyses. Four amino acid changes were observed among SLA-DQA haplotype c, d and DQA-SLT 26. They were Val-->Ala(60), Lys-->Glu(65), Asp-->Gly(81) and Lys-->Ile(93). Comparing the amino acids sequence of the all SLA-DQA sequences with DQA-TC 21-1 revealed that the His (94) was changed into Tyr.