AI Article Synopsis
- Sensitive assays are essential for detecting bovine leukemia virus (BLV) in donor cattle for Australian tick fever vaccines.
- The study developed a sensitive 5' Taq nuclease assay using specific DNA probes and compared it with traditional PCR methods.
- The TaqMan MGB assay proved to be the most sensitive and specific for BLV detection among various tested methods, marking a significant advancement in the field.
Article Abstract
Sensitive assays are required to detect proviral bovine leukemia virus (BLV) in donor cattle used for the in vivo preparation of Australian tick fever vaccines. 5' Taq nuclease assays using 3' minor groove binder DNA probes (TaqManMGB) were developed and compared to conventional PCR assays for sensitive detection of Australian BLV. Seven beef and dairy herds were screened using DNA prepared by a variety of protocols to evaluate these tests. Comparative sensitivities of PCR tests were determined by testing log(10) dilutions of plasmids with inserted BLV sequences. Animals were also screened by the BLV standard agar-gel immunodiffusion test (AGID) and commercial enzyme linked immunosorbent assays (ELISA) for antibodies, and an ELISA for detecting viral antigens expressed (VAE) in lymphocyte cultures. The TaqMan MGB assay based on the pol region was the most sensitive and specific for the detection of BLV. This is the first report of a sensitive BLV 5' Taq nuclease assay.
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| http://dx.doi.org/10.1016/j.jviromet.2003.09.029 | DOI Listing |
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