AI Article Synopsis
- The study aimed to explore how apoptosis (cell death) relates to the expression of the Fas gene in oral squamous cell carcinoma.
- The researchers used a plasmid to introduce the Fas gene into Tca8113 cells and measured changes in both mRNA and protein levels of Fas, finding increased expressions after transfection.
- Results indicated that while the rate of Fas protein expression didn't change significantly, its intensity and the rate of apoptosis increased, suggesting a threshold may exist before apoptosis is triggered in these cancer cells.
Article Abstract
Objective: To investigate the relationship of apoptosis and expression of Fas gene in oral squamous cell carcinoma.
Methods: Plasmid including Fas gene was transfected into Tca8113 cell by lipofectamine kit. The expression of Fas mRNA in transfected and non-transfected cell was detected by RT-PCR. The expression of Fas protein and apoptosis index(AI) was assessed by flow cytometry(FCM).
Results: No variation of positive expression rate of Fas protein was found in Fas-transfected and non-transfected Tca8113 (P > 0.05), but the expression of Fas mRNA, intensity of Fas protein and apoptosis index were significantly increased respectively from 0.201 +/- 0.015, 31.02 +/- 4.63, 0.88% +/- 0.16% to 0.399 +/- 0.073, 54.32 +/- 6.38 and 10.21% +/- 1.46%(P < 0.01).
Conclusion: Apoptosis in tongue squamous cell carcinoma was not directly associated with the positive expression rate but intensity of Fas protein. A liminal value might exist before apoptosis in Tca8113 cell could be activated.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Fluorescent antennas (FAs) exhibit considerable promise in optical wireless communication (OWC), primarily due to their advantages over conventional optical systems in terms of optical gain and field of view (FoV). This paper presents a COMSOL-based model designed to optimize external light-concentrating structures for FAs, with its accuracy validated through both qualitative and quantitative comparisons. Leveraging refractive index modulation and the conservation of optical étendue, two distinct light-concentrating structures are developed.
View Article and Find Full Text PDFSilibinin, a PLC-β3 inhibitor, inhibits mast cell activation and alleviates OVA-induced asthma.
Mol Immunol
January 2025
Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan; Master Program of Pharmaceutical Manufacture, College of Pharmacy, China Medical University, Taichung, Taiwan; Department of Medical Laboratory Science and Biotechnology, College of Medical and Health Science, Asia University, Taichung, Taiwan. Electronic address:
The immunoglobulin E (IgE) receptor FcεRI (Fc epsilon RI) plays a crucial role in allergic reactions. Recent studies have indicated that the interaction between FcεRIβ and the downstream protein phospholipase C beta 3 (PLCβ3) leads to the production of inflammatory cytokines. The aim of this study was to develop small molecules that inhibit the protein-protein interactions between FcεRIβ and PLCβ3 to treat allergic inflammation.
View Article and Find Full Text PDFUrine proteomics defines an immune checkpoint-associated nephritis signature.
J Immunother Cancer
January 2025
Section of Nephrology, Division of Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Immune checkpoint inhibitor (ICI) therapy is a cornerstone treatment for many cancers, but it can induce severe immunotoxicity, including acute interstitial nephritis (AIN). Currently, kidney biopsy is required to differentiate ICI-AIN from other causes of acute kidney injury (AKI). However, this invasive approach can lead to morbidity, delayed glucocorticoid treatment for patients with AIN, and unnecessarily prolonged suspension of ICI therapy in non-AIN patients.
View Article and Find Full Text PDFDengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation.
J Biol Chem
January 2025
Institute of Virology, Philipps University Marburg, Marburg, Germany. Electronic address:
Orthoflaviviruses are emerging arthropod-borne pathogens whose replication cycle is tightly linked to host lipid metabolism. Previous lipidomic studies demonstrated that infection with the closely related hepatitis C virus (HCV) changes the fatty acid (FA) profile of several lipid classes. Lipids in HCV-infected cells had more very long-chain and desaturated FAs and viral replication relied on functional FA elongation and desaturation.
View Article and Find Full Text PDFExperimental Validation of Antiobesogenic and Osteoprotective Efficacy of Ginsenoside CK via Targeting Lipid and Atherosclerosis Pathways.
Life (Basel)
December 2024
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea.
The present study explored the possible antiobesogenic and osteoprotective properties of the gut metabolite ginsenoside CK to clarify its influence on lipid and atherosclerosis pathways, thereby validating previously published hypotheses. These hypotheses were validated by harvesting and cultivating 3T3-L1 and MC3T3-E1 in adipogenic and osteogenic media with varying concentrations of CK. We assessed the differentiation of adipocytes and osteoblasts in these cell lines by applying the most effective doses of CK that we initially selected.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!