[Research of immunization with recombinant plasmid pcDNA3-pacA and pcDNA3-pacP against dental caries in gnotobiotic rats. I: Measurement of specific anti-PAc S-IgA, IgG, valence].

Objective: In this study, rats were immunized with pcDNA3-pacA and pcDNA3-pacP through submandibular gland, the valence of specific anti-PAc S-IgA,IgG were measured.

Methods: Gnotobiotic rats were vaccinated with pcDNA3-pacA, pcDNA3-pacP and combination use of pcDNA3-pacA and pcDNA3-pacP through submandibular gland. The valence of specific anti-PAc S-IgA in salivary, IgG in serum were measured.

Results: The specific anti-PAc S-IgA, IgG could be detected in all of recombinant plasmid groups. The level of salivary specific anti-PAc S-IgA and IgG in pcDNA3-pacA, pcDNA3-pacP, combination use of pcDNA3-pacA and pcDNA3-pacP groups were higher than negative control groups (P < 0.05).

Conclusion: The recombinant plasmid pcDNA3-pacA and pcDNA3-pacP could provoke specific mucosal immune responses as a novel immunogen against dental caries.