Granulocytic differentiation of myeloid NB4 cells was noted to transactivate the genomic human growth hormone (hGH) reporter construct in the absence of a driving test promoter. Such spurious hGH transactivation also occurred upon co-transfection of plasmids expressing the transactivators c-Myb and C/EBPalpha into undifferentiated NB4 cells. Modifications to the reporter gene and the vector resulted in a reporter construct unaffected by such stimuli. When human neutrophil peptide 1 (hnp-1) gene promoter fragments were tested using both the original and modified reporter constructs, a clear difference in the pattern of transactivation was noted. This suggests that promoter and enhancer elements in the reporter construct plasmid DNA can effect the analysis of the test promoter.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gene.2003.09.020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of glycogen synthase kinase 3alpha/beta as a host factor required for hepatitis B virus transcription using high-throughput screening.
Hepatology
January 2025
Genome Medical Science Project, National Center for Global Health and Medicine, Ichikawa, Japan.
Background Aims: Hepatitis B virus (HBV) leads to severe liver diseases, such as cirrhosis and hepatocellular carcinoma. Identification of host factors that regulate HBV replication can provide new therapeutic targets. The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV entry receptor has enabled the establishment of hepatic cell lines for analyzing HBV infection and propagation.
View Article and Find Full Text PDFGeneration of ribosomal protein S1 mutants for improving of expression of difficult to translate mRNAs.
Appl Microbiol Biotechnol
January 2025
Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland.
Metagenomes present a source for novel enzymes, but under 1% of environmental microbes are cultivatable. Because of its useful properties, Escherichia coli has been used as a host organism in functional genomic screens. However, due to differing expression machineries in the expression host compared to the source organism of the DNA sequences, screening outcomes can be biased.
View Article and Find Full Text PDFm6A Demethylase -Mediated Upregulation of Induces Neuronal Ferroptosis via the Axis in the MPP/MPTP-Induced Parkinson's Disease Model.
ACS Chem Neurosci
January 2025
Department of Neurology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China.
: Parkinson's disease (PD) is a neurodegenerative disorder characterized by the involvement of ferroptosis in its pathological mechanism. In this study, the effects and mechanism of BRCA1-associated protein 1 (BAP1) on neuronal ferroptosis in PD were evaluated. : A PD mouse model was constructed by injecting mice with MPTP.
View Article and Find Full Text PDFClinical Diagnostic Value of miR-193a-5p in Neonatal Acute Respiratory Distress Syndrome and Analysis of Its Effect on Human Lung Epithelial Cells.
Fetal Pediatr Pathol
January 2025
Department of Respiratory and Critical Care Medicine, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, China.
: To explore the clinical value of miR-193a-5p in neonatal acute respiratory distress syndrome (ARDS) and its role in ARDS cell model . : RT-qPCR was utilized to detect miR-193a-5p level. Correlation analysis was implemented to assess the correlation between miR-193a-5p and clinical indicators (IL-6, IL-1β, TNF-α, LUS).
View Article and Find Full Text PDFGenetic predisposition to Behcet's disease mediated by a IL10RA enhancer polymorphism.
Heliyon
January 2025
The First Affiliated Hospital of Chongqing Medical University, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing, PR China.
Background: Several studies suggested the genetic association between IL10RA variants and susceptibility to Behcet's disease (BD). However, the precise mechanism of the association is still unknown. The purpose of this study was to investigate the mechanism underlying the genetic associations between IL10RA polymorphisms and the risk of BD.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!