Two-dimensional imaging biosensor for the monitoring of lactate released from brain slices.

Real-time monitoring of lactate release from brain slices has been studied with an optical two-dimensional (2D) imaging biosensor. The 2D biosensor is prepared by direct immobilization of lactate dehydrogenase (LDH) molecules onto a flat silica glass surface through a covalent binding mechanism. The biosensor is able to spatially differentiate lactate concentration variations with conventional optical microscopic spatial resolution. This biosensor has the capability to effectively detect lactate down to a concentration of 100 nM. The 2D biosensor responds uniformly with 2.5% RSD from pixel to pixel. With a 100 ms response time, this 2D biosensor has the capability of monitoring simultaneously many cells in one image. We have studied the impact of KCI on lactate release from brain slices. Clear differences have been observed in lactate release for different regions of the tissue. The real-time determination of the newly released lactate from the mouse brain slices clearly demonstrates the feasibility of monitoring lactate release from living specimens. The 2D biosensor will enable us to study cellular communications and possibly other biological processes that require simultaneous temporal and spatial resolution.