AI Article Synopsis
- The Loop-mediated isothermal amplification (LAMP) method is a fast and highly sensitive technique used for diagnosing diseases in aquaculture, particularly for detecting the white spot syndrome virus (WSSV) in shrimp.
- Researchers designed a specific diagnostic system using four primers from the WSSV genome and optimized the reaction conditions to 60 minutes at 65°C.
- The LAMP method proved to be more sensitive than traditional nested PCR, with a detection limit of 1 fg compared to 10 fg, making it an effective tool for identifying WSSV in various shrimp tissues.
Article Abstract
Loop-mediated isothermal amplification (LAMP) method is a novel, sensitive and rapid technique which can be applied for disease diagnosis in aquaculture. Using the LAMP method, a highly specific and sensitive diagnostic system for white spot syndrome virus (WSSV) detection was designed. A set of four primers, two outer and two inner primers, were designed from WSSV genome DNA. Reaction time and temperatures were optimized for 60 min at 65 degrees C, respectively. The detection limit (DL) using the LAMP method was up to 1 fg, when compared to 10 fg by nested polymerase chain reaction (PCR). Thus, standardized LAMP procedure was used to detect the presence of WSSV in the heart, stomach and lymphoid organ from infected shrimp. The study has developed a diagnostic procedure which is a rapid and highly sensitive for WSSV detection in shrimp.
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| http://dx.doi.org/10.1016/j.jviromet.2003.09.015 | DOI Listing |
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