AI Article Synopsis
- Telomerase is an enzyme that helps maintain the protective ends of chromosomes by adding DNA sequences known as telomeric repeats.
- One of its critical components, hTERT, has multiple isoforms due to alternative splicing, but their expression in gastrointestinal cells was previously unknown.
- A study developed a PCR test that found specific hTERT variants common in gastric and liver cancer cell lines but absent in colorectal cancer cell lines, offering insights into telomerase regulation in these cancers.
Article Abstract
Telomerase is a cellular RNA-dependent DNA polymerase that serves to maintain the tandem arrays of telomeric TTAGGG repeats at eukaryotic chromosome ends. One of the human telomerase components is hTERT, which has three alternative spliced sites that introduce eight isoforms of hTERT mRNA. The expression of these isoforms in gastrointestinal cell lines is unknown. We developed a PCR-based assay for detecting these splicing variants. In gastric and hepatocellular carcinoma cell lines, the gamma deletion variant and its combination variants, alpha- and gamma-, beta- and gamma-, and alpha-, beta- and gamma-deletion variants were frequently detected, while they were not detected in colorectal carcinoma cell lines. Our results provide important information of use for more detailed studies on the regulation of telomerase activity.
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