AI Article Synopsis
- The study identified and cloned tdn genes from Frateuria sp. ANA-18, which are responsible for degrading aniline through the ortho-cleavage pathway of catechol.
- These tdn genes are distinct from the genes that degrade aniline in Pseudomonas and Acinetobacter, which use a different pathway (meta-cleavage) and organize their genes differently.
- Disruption of tdn genes led to a loss of aniline degradation in strain ANA-18, confirming that these genes are essential for the bacterium's ability to metabolize aniline.
Article Abstract
Genes encoding an aniline dioxygenase of Frateuria sp. ANA-18, which metabolizes aniline via the ortho-cleavage pathway of catechol, were cloned and named tdn genes. The tdn genes were located on the chromosomal DNA of this bacterium and weren't clustered with catechol-degrading gene clusters. These results show that the ANA-18 aniline-degrading gene cluster is constructionally different from Pseudomonas tdn and Acinetobacter atd gene clusters, which degrade aniline via the meta-cleavage pathway of catechol and organize catechol-metabolic genes in the gene clusters. When cloned tdnQTA1A2B genes were expressed in Eschherichia coli, aniline dioxygenase activity was observed. Southern blot analysis revealed that homologues of the tdnA1A2B genes didn't exist in strain ANA-18. Disruption of the tdnA1A2 genes gave the parent strain ANA-18 a defect in aniline metabolism. On the basis of these results, we concluded that only the cloned tdn genes function as genes encoding aniline dioxygenase in strain ANA-18 although this bacterium had two catechol-degrading gene clusters.
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| http://dx.doi.org/10.1271/bbb.67.2351 | DOI Listing |
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