A curcin gene was isolated by using genomic walker technology and revealed to encode the type-1 ribosome inactivating proteins (RIPs). Analysis of 1802 bp segments revealed the gene including a 694 bp 5' flanking region, a 882 bp open read frame (ORF) and a 226 bp 3' flanking region. There are one putative TATA boxes and two possible CAAT box lie in the 5'-flanking region. The ORF encodes a 32 kDa precursor, which contains a 42 amino acid signal peptide. Two possible polyadenylation signals are found in the 3'-flanking region. No introns were found, which is typical of other RIPs gene that has been sequenced. The deduced amino acid sequence of Curcin gene coding region shares high homology with RIPs, e.g. ricin A-chain, gelonin, abrin A-chain, bryodin, trichosanthin and momorcharin, which were found to be 34% (99/287), 34% (98/287), 37% (89/240), 34% (86/249), 36% (87/241) and 36% (88/241), respectively. The cloning of the gene is important foundation to further study the structure, expression and regulation mechanism.
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http://dx.doi.org/10.1080/1042517031000119348 | DOI Listing |
Learn Health Syst
January 2024
Interdisciplinary Bank of Biomaterials and Data Würzburg (ibdw) Würzburg Germany.
Toxins (Basel)
November 2022
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610041, China.
Curcin and Curcin C, both of the ribosome-inactivating proteins of , have apparent inhibitory effects on the proliferation of osteosarcoma cell line U20S. However, the inhibitory effect of the latter is 13-fold higher than that of Curcin. The mechanism responsible for the difference has not been studied.
View Article and Find Full Text PDFBMC Plant Biol
October 2015
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Republic of Singapore.
Background: Jatropha curcas L. is a potential biofuel plant and its seed oil is suitable for biodiesel production. Despite this promising application, jatropha seeds contain two major toxic components, namely phorbol esters and curcins.
View Article and Find Full Text PDFBiotechnol J
April 2015
Plant Biotechnology Unit (PBU), Department of Biotechnology, BOKU-VIBT, University of Natural Resources and Life Sciences, Vienna, Austria.
Increasing economic interest in Jatropha curcas requires a major research focus on the genetic background and geographic origin of this non-edible biofuel crop. To determine the worldwide genetic structure of this species, amplified fragment length polymorphisms, inter simple sequence repeats, and novel single nucleotide polymorphisms (SNPs) were employed for a large collection of 907 J. curcas accessions and related species (RS) from three continents, 15 countries and 53 regions.
View Article and Find Full Text PDFMol Biol Rep
July 2014
Defence Institute of Bio-Energy Research, Haldwani-263 139, Nainital, Uttarakhand, India,
Curcin, a type I ribosomal inhibiting protein-RIP, encoded by curcin precursor gene, is a phytotoxin present in Jatropha (Jatropha curcas L.). Here, we report designing of RNAi construct for the curcin precursor gene and further its genetic transformation of Jatropha to reduce its transcript expression.
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