AI Article Synopsis
- The study presents a new method for quickly mapping disease-related chromosomal translocation breakpoints using flow-sorted chromosomes and array-based comparative genomic hybridization (arrayCGH).
- To validate this method, researchers used a specific cell line with known chromosomal breakpoints, successfully identifying exact locations on chromosomes 12 and 15 in one experiment.
- The research also determined that reliable results can be obtained with as few as 10,000 flow-sorted chromosomes, making the approach efficient and practical.
Article Abstract
Despite the recent completion of the human genome project, the mapping of disease-related chromosomal translocation breakpoints and genes has remained laborious. Here, we describe a novel and rapid procedure to map such translocation breakpoints using flow-sorted chromosomes in combination with array-based comparative genomic hybridization (arrayCGH). To test the feasibility of this approach, we used a t(12;15)(q13;q25)-positive cell line with known breakpoint positions as a model. The derivative 12 chromosomes were flow-sorted, labeled, and hybridized to a genome-wide array containing 3648 well-characterized human genomic clones. The exact locations of the breakpoints on both chromosome 12 and 15 could be determined in a single hybridization experiment. In addition, we have tested the minimal amount of material necessary to perform these experiments and show that it is possible to obtain highly reliable profiles using as little as 10,000 flow-sorted chromosomes.
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| http://dx.doi.org/10.2144/03355dd03 | DOI Listing |
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