A thermophilic bacterium, identified as a neighboring species to Geobacillus thermocatenulatus, having a growth optimum at 55 degrees C and, capable of degrading nylon 12, was isolated from soil by enrichment culture technique at 60 degrees C. At this temperature, the strain grew on 5 g nylon 12 l(-1) with a decrease in its molecular weight from 41000 to 11000 over 20 d. The degradation was assumed to be due to endogenous hydrolysis of amide bond in nylon 12. The strain degraded also nylon 66 with a decrease in its molecular weight from 43000 to 17000 in 20 d at 60 degrees C. Nylon 6 was not degraded.
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http://dx.doi.org/10.1023/a:1026091711130 | DOI Listing |
Chemosphere
January 2025
BioEngine Research team on green process engineering and biorefineries, Chemical Engineering Department, Université Laval, Pavillon Adrien-Pouliot 1065, av. de la Médecine Québec (Québec), Canada; CentrEau, Centre de recherche sur l'eau, Université Laval, 1065 Avenue de la Médecine, Québec, QC, G1V 0A6, Canada. Electronic address:
The role of inoculum in initiating anaerobic digestion (AD), and accelerating the start-up of anaerobic digesters has been well-documented. However, the effect of aligning the origin temperature of the inoculum with the operational temperature of the new digester remains underexplored. This study investigates how the origin temperature and characteristics of the inoculum affect the kinetics and biodegradability of sewage sludge (SS) and microcrystalline cellulose (MCC) under mesophilic and thermophilic conditions.
View Article and Find Full Text PDFBioresour Technol
January 2025
Laboratorio de Investigación en Procesos Avanzados de Tratamiento de Aguas, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Querétaro 76230, México. Electronic address:
Microalgae-bacteria-based systems are an emerging and promising approach for wastewater treatment plants (WWTP), having nutrient and antibiotic resistance removal comparable to conventional technologies. Still, antibiotic-resistance genes and bacteria (ARG and ARB) can proliferate in microalga-bacteria aggregates (MABA), a concern to control. Different temperature regimes of MABA continuous anaerobic digestion (AD), thermophilic (55 °C), and mesophilic (35 °C) were evaluated in this study as a strategy to eliminate ARB and ARGs.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.
Thermophilic microbial communities growing in low-oxygen environments often contain early-evolved archaea and bacteria, which hold clues regarding mechanisms of cellular respiration relevant to early life. Here, we conducted replicate metagenomic, metatranscriptomic, microscopic, and geochemical analyses on two hyperthermophilic (82-84 °C) filamentous microbial communities (Conch and Octopus Springs, Yellowstone National Park, WY) to understand the role of oxygen, sulfur, and arsenic in energy conservation and community composition. We report that hyperthermophiles within the Aquificota (Thermocrinis), Pyropristinus (Caldipriscus), and Thermoproteota (Pyrobaculum) are abundant in both communities; however, higher oxygen results in a greater diversity of aerobic heterotrophs.
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
December 2024
Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.
A thermophilic cellulase-producing bacterium, strain HSW-8, isolated from hot spring waters in South Korea, was subjected to a taxonomic analysis. Cells of strain HSW-8 were gram-stain-negative, facultatively anaerobic, rod-shaped, with optimum growth at 45 °C, pH 7.0, in the presence of 0% (w/v) NaCl.
View Article and Find Full Text PDFBMC Microbiol
December 2024
Winogradsky Institute of Microbiology Federal Research Center Fundamentals of Biotechnology Russian Academy of Sciences, 60 let Oktyabrya Prospect, 7 Build.2, Moscow, Russia.
The Kuril Islands are located in the Far-East of Russia and enriched with shallow and terrestrial hot springs. Prokaryotic diversity of Kuril geothermal environments has been studied fragmentarily and mainly by culture-dependent methods. We performed the first large-scale investigation of microbial communities, inhabited more than 30 terrestrial hot springs of Kunashir and Iturup Islands, analyzed by 16S rRNA gene fragment amplicon sequencing, together with chemical analysis of thermal waters and sediments.
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