Objective: To transfect nm23-H1 into the Acc-M cell lines in a safe, high-efficiency and low-toxicity way, and then to find out whether nm23-H1 affects the metastasis ability and chemo-sensitivity of Acc-M cell lines.
Methods: Lipofect was used to transfect nm23-H1 into Acc-M cell lines. The difference in expression of nm23-H1 between the transfected and non-transfected cell lines was detected by immunohistochemistry. Then by use of transwell-room and wash way, the difference in invasion and metastasis ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of chemo-sensitivity.
Results: Using pCMV-Neo-Bam system, we observed the stable expression of nm23-H1 and the significant difference in Nucleoside Diphosphate kinase-A, expression between the transfected and non-transfected Acc-M cell lines. The metastasis ability of transfected Acc-M cell lines decreased significantly. The chemo-sensitivity of transfected Acc-M cell line to cis-diamminedichloroplatin (C-DDP) increased significantly.
Conclusion: nm23-H1 can inhibit the metastases of Acc-M cell lines significantly and can increase the chemo-sensitivity to C-DDP significantly.
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