[Research of the amounts of flavonoids accumulated in the crude drug of single-styled hawthorn].

Unlabelled: Leaves, flowers and fruits of single-styled hawthorn are used for production of pharmaceuticals with cardiotonic, coronary vasodilatoric and hypotensive action. Amount of flavonoids accumulated in crude drug of hawthorn is a parameter of quality.

Objective: To establish the difference in amounts of flavonoids accumulated in the crude drug of single-styled hawthorn.

Materials And Methods: Different examples of crude material from some Lithuanian regions were extracted with ethanol; flavonoids were measured by spectroscopic method and by HPLC (high performance liquid chromatography).

Results: From 0.77% to 2.80% of flavonoids are determined in the leaves and flowers; amounts of hyperosid were 0.16-0.87%; vitexin-o-rhamnosid 0.14-0.40%; rutin 0.10-0.19%. Flavonoids maximum percent was determinate in crude material examples collected at the single-styled hawthorn flowering time; the decline of amounts of flavonoids accumulated in the crude material of hawthorn was determined from May to August (realization of correlation coefficient r=-0.90). Hyperosid maximum percent was determined in crude material examples collected at flowering time; the decline of amounts of hyperosid accumulated in the crude material was determined from May to August (r=-0,94). Amounts of rutin and vitexin-o-rhamnosid in leaves were less than flowers; there was the decline of amounts of rutin and vitexin-o-rhamnosid in leaves from May to August. The percent of flavonoids 0.09 to 0.24 is determined in fruits.

Conclusion: Lithuanian single-styled hawthorn populations can supply quality crude material from May to August; the bigger amounts of flavonoids were determined in crude drug examples collected at flowering time.