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Functional reconstitution of plant plasma membrane H-ATPase into giant unilamellar vesicles.
Sci Rep
March 2025
Department of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany.
Membrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges. Reconstitution of membrane transporters in model systems offers a powerful solution.
View Article and Find Full Text PDFIncorporation of Triacylglycerol and Cholesteryl Ester Droplets in Phase-Separated Giant Unilamellar Vesicles.
Langmuir
March 2025
Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Cytoplasmic lipid droplets form from the endoplasmic reticulum (ER). Because the ER membrane can undergo phase separation, the interaction of lipid droplets with phase-separated bilayers is of significant interest. In this study, we used fluorescence microscopy to investigate the incorporation of droplets composed of triolein, trilinolein, trimyristolein, trieicosenoin, and cholesteryl arachidonate in the bilayers of giant unilamellar vesicles (GUVs) consisting of a mixture of 1,2-dioleoyl--glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl--glycero-3-phosphocholine (DPPC), and cholesterol.
View Article and Find Full Text PDFMembrane Fusion-Based Drug Delivery Liposomes Transiently Modify the Material Properties of Synthetic and Biological Membranes.
Small
February 2025
Moleculaire Biofysica, Zernike Instituut, Rijksuniversiteit Groningen, Groningen, The Netherlands.
Many drug targets are located in intracellular compartments of cells but they often remain inaccessible to standard imaging and therapeutic agents. To aid intracellular delivery, drug carrier nanoparticles have been used to overcome the barrier imposed by the plasma membrane. The carrier must entrap large amounts of cargo, efficiently and quickly deliver the cargo in the cytosol or other intracellular compartments, and must be inert; they should not induce cellular responses or alter the cell state in the course of delivery.
View Article and Find Full Text PDFPepticombisomes: Biomimetic Vesicles Crafted From Recombinant Supercharged Polypeptides with Uniformly Distributed Side-Chains.
Adv Sci (Weinh)
February 2025
Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 2, 52074, Aachen, Germany.
Cell membranes play a key role in bottom-up synthetic biology, as they enable interaction control, transport, and other essential functions. These ultra-thin, flexible, yet stable structures form through the self-assembly of lipids and proteins. While liposomes are common mimics, their synthetic membranes often fail to replicate natural properties due to poor structural control.
View Article and Find Full Text PDFLiposome-Encapsulated Lysates to Reconstitute Intracellular Macromolecular Crowding Effects.
ACS Synth Biol
February 2025
Cellular Protein Chemistry, Bijvoet Centre for Biomolecular Research, Faculty of Science, Utrecht University, Utrecht 3584 CH, The Netherlands.
Intracellular macromolecular crowding impacts biomacromolecule behavior, including oligomerization, phase separation, and diffusion. However, understanding crowding effects in cells is challenging as cells respond and adapt to perturbations. Therefore, replicating in-cell crowding in liposomes would provide a good alternative to studying the consequences of macromolecular crowding.
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