Objective: To determine the effect of the fusion of prostasomes to spermatozoa on the acrosome reaction.
Design: In vitro study of human spermatozoa.
Setting: Healthy volunteers in an academic research environment.
Patient(s): Healthy volunteer men, 25 to 35 years old.
Intervention(s): Human semen was fractionated into spermatozoa and prostasomes. Fusion of prostasome to spermatozoa was performed at pH 5.5. Progesterone (1 microM) was added when required.
Main Outcome Measure(s): Evaluation of the acrosome reaction by fluorescence microscopy.
Results(s): The percentage of spontaneously acrosome-reacted cells was very low unless the Ca(2+)-ionophore A 23187 was added. The treatment of spermatozoa with 1 microM of progesterone scarcely affected the acrosome reaction; a pretreatment in conditions permitting fusion increased it. The addition of progesterone to prostasome-fused spermatozoa further increased the extent of the acrosome reaction.
Conclusion(s): The H(+)-dependent fusion with prostasomes makes spermatozoa more sensitive to the effect of progesterone on acrosome-reaction induction.
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