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Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. | LitMetric

Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR.

J Clin Microbiol

Department of Pathology, School of Medicine, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15213, USA.

Published: November 2003

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0.900), whereas the plasma and PBL loads correlated poorly (r(2) = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC262487PMC
http://dx.doi.org/10.1128/JCM.41.11.5245-5249.2003DOI Listing

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